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〈論文〉siRNA-NESコンジュゲートの合成、デリバリー、遺伝子サイレンシング
https://doi.org/10.15100/0000011526
https://doi.org/10.15100/0000011526c34c3b2d-ab7c-40cf-8a22-8d1bf41fc249
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA12013209-20111210-0001.pdf (1.4 MB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||
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| 公開日 | 2012-01-30 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | 〈論文〉siRNA-NESコンジュゲートの合成、デリバリー、遺伝子サイレンシング | |||||||||||||||
| 言語 | ja | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | 〈PAPER〉Synthesis, Delivery and Gene Silencing of siRNA-NES Conjugates | |||||||||||||||
| 言語 | en | |||||||||||||||
| 著者 |
藤井, 政幸
× 藤井, 政幸
× Diala, Irmina
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| 言語 | ||||||||||||||||
| 言語 | jpn | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | siRNA, nuclear export signal (NES) peptides, solid phase fragment condensation, cellular delivery, gene silencing, human telomerase elongation reverse transcriptase (hTERT) 核外輸送シグナルペプチド, 固相フラグメント縮合法, 細胞内デリバリー, 遺伝子サイレンシング, ヒトテロメラーゼ逆転写酵素 |
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| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||
| ID登録 | ||||||||||||||||
| ID登録 | 10.15100/0000011526 | |||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||
| 著者 所属 | ||||||||||||||||
| 値 | 近畿大学産業理工学部生物環境化学科; 教授 | |||||||||||||||
| 著者 所属 | ||||||||||||||||
| 値 | 近畿大学大学院産業技術研究科物質工学専攻博士後期課程 | |||||||||||||||
| 著者所属(翻訳) | ||||||||||||||||
| 値 | Faculty of Humanity-Oriented Science and Engineering, Kinki University | |||||||||||||||
| 版 | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
| 出版者 名前 | ||||||||||||||||
| 出版者 | 近畿大学産業理工学部 | |||||||||||||||
| 書誌情報 |
ja : かやのもり：近畿大学産業理工学部研究報告 en : Reports of School of Humanity-Oriented Science and Engineering, Kinki University 号 15, p. 1-6, 発行日 2011-12-01 |
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| ISSN | ||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 13495801 | |||||||||||||||
| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | Herein we described the synthesis of siRNA-NES (nuclear export signal) peptide conjugates by solid phase fragment coupling (SPFC) and application of them to silencing of bcr/abl chimeric gene in human leukemia cell line K562. Synthesis of siRNA-NES conjugates was achieved by SPFC. As a result, two types of siRNA-NES conjugates C1 and C2 were prepared in which 5' -end of sense strand was covalently linked to N-terminus of the NES peptides derived from TFIIIA and HIV-1rev, respectively. Silencing effects of C1 and C2 against bcr/abl mRNA in human leukemia cell line K562 were evaluated by quantitative PCR. The expression of bcr/abl gene was suppressed to 30.2％ at 200nM and 36.3％ at 50 nM by native siRNA. Significant enhancement of silencing efficiency was observed with C1 and C2. siRNA-TFIIIA NES (C1) suppressed the expression of bcr/abl gene to 8.3％ at 200 nM and 11.6％ at 50nM and siRNA-HIV-1rev NES (C2) suppressed to 4.0％ at 200 nM and 6.3％ at 50nM. Previously, we reported that DNA-HIV-1 rev NES peptide conjugate was localized in cytoplasm of Jurkat cell. The large enhancement of the silencing efficiency of siRNA-NES conjugates could be reasonably ascribed to the localization of siRNA-NES conjugates in cytoplasm. It can be also pointed out that modification of 5' -endo of sense strand reduced off-target effect by minimizing the extent of the sense strand incorporation into RISC. (3) Unfortunately, siRNA-NES conjugates could not penetrate the cellular membrane by itself and required a transfection reagent to be taken up into cells, while oligodeoxyribonucleotide-NES conjugates were internalized into cells without any transfection reagents. It can be elucidated the double stranded structure of siRNA retarded the penetration through cellular membrane. | |||||||||||||||
| 言語 | en | |||||||||||||||
| フォーマット | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||
