{"created":"2023-06-20T16:41:06.786554+00:00","id":11526,"links":{},"metadata":{"_buckets":{"deposit":"062a70d4-52a7-42f7-ae98-4b42143c06cd"},"_deposit":{"created_by":3,"id":"11526","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"11526"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00011526","sets":["14:923:1809:1825","21:3039:3610:3616"]},"author_link":["22164","22165"],"control_number":"11526","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"かやのもり：近畿大学産業理工学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Reports of School of Humanity-Oriented Science and Engineering, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Herein we described the synthesis of siRNA-NES (nuclear export signal) peptide conjugates by solid phase fragment coupling (SPFC) and application of them to silencing of bcr/abl chimeric gene in human leukemia cell line K562. Synthesis of siRNA-NES conjugates was achieved by SPFC. As a result, two types of siRNA-NES conjugates C1 and C2 were prepared in which 5' -end of sense strand was covalently linked to N-terminus of the NES peptides derived from TFIIIA and HIV-1rev, respectively. Silencing effects of C1 and C2 against bcr/abl mRNA in human leukemia cell line K562 were evaluated by quantitative PCR. The expression of bcr/abl gene was suppressed to 30.2％ at 200nM and 36.3％ at 50 nM by native siRNA. Significant enhancement of silencing efficiency was observed with C1 and C2. siRNA-TFIIIA NES (C1) suppressed the expression of bcr/abl gene to 8.3％ at 200 nM and 11.6％ at 50nM and siRNA-HIV-1rev NES (C2) suppressed to 4.0％ at 200 nM and 6.3％ at 50nM. Previously, we reported that DNA-HIV-1 rev NES peptide conjugate was localized in cytoplasm of Jurkat cell. The large enhancement of the silencing efficiency of siRNA-NES conjugates could be reasonably ascribed to the localization of siRNA-NES conjugates in cytoplasm. It can be also pointed out that modification of 5' -endo of sense strand reduced off-target effect by minimizing the extent of the sense strand incorporation into RISC. (3) Unfortunately, siRNA-NES conjugates could not penetrate the cellular membrane by itself and required a transfection reagent to be taken up into cells, while oligodeoxyribonucleotide-NES conjugates were internalized into cells without any transfection reagents. It can be elucidated the double stranded structure of siRNA retarded the penetration through cellular membrane.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15100/0000011526","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学産業理工学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13495801","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 教授"},{"subitem_text_value":"近畿大学大学院産業技術研究科物質工学専攻博士後期課程"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Faculty of Humanity-Oriented Science and Engineering, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤井,  政幸","creatorNameLang":"ja"},{"creatorName":"フジイ, マサユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Diala,  Irmina","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-19"}],"displaytype":"detail","filename":"AA12013209-20111210-0001.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA12013209-20111210-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/11526/files/AA12013209-20111210-0001.pdf"},"version_id":"f0a26352-c467-41c0-afad-949269d8912d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"siRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"核外輸送シグナルペプチド","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"固相フラグメント縮合法","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞内デリバリー","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子サイレンシング","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒトテロメラーゼ逆転写酵素","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"nuclear export signal (NES) peptides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"solid phase fragment condensation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cellular delivery","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene silencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human telomerase elongation reverse transcriptase (hTERT)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈論文〉siRNA-NESコンジュゲートの合成、デリバリー、遺伝子サイレンシング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈論文〉siRNA-NESコンジュゲートの合成、デリバリー、遺伝子サイレンシング","subitem_title_language":"ja"},{"subitem_title":"〈PAPER〉Synthesis, Delivery and Gene Silencing of siRNA-NES Conjugates","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["1825","3616"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-01-30"},"publish_date":"2012-01-30","publish_status":"0","recid":"11526","relation_version_is_last":true,"title":["〈論文〉siRNA-NESコンジュゲートの合成、デリバリー、遺伝子サイレンシング"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-08T00:35:18.552300+00:00"}