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Burkholderia cepaciaからクローニングしたキチナーゼ遺伝子の塩基配列決定
https://kindai.repo.nii.ac.jp/records/9845
https://kindai.repo.nii.ac.jp/records/98459d84d4cf-5f4b-46a0-bd98-d23760e9de76
名前 / ファイル | ライセンス | アクション |
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AN10410378-20010315-0129.pdf (1.1 MB)
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Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2011-11-21 | |||||
タイトル | ||||||
タイトル | Burkholderia cepaciaからクローニングしたキチナーゼ遺伝子の塩基配列決定 | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | DNA sequence of chitinase gene cloned from Burkholderia cepacia | |||||
著者 |
松田, 克礼
× 松田, 克礼× 豊田, 秀吉× 野々村, 照雄× 角谷, 晃司× 池田, 成志× 玉井, 隆行× 桑原, 精宏× 大内, 成志 |
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言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者(英) | ||||||
en | ||||||
Matsuda, Yoshinori | ||||||
著者(英) | ||||||
en | ||||||
Toyoda, Hideyoshi | ||||||
著者(英) | ||||||
en | ||||||
Nonomura, Teruo | ||||||
著者(英) | ||||||
en | ||||||
Kakutani, Koji | ||||||
著者(英) | ||||||
en | ||||||
Ikeda, Seishi | ||||||
著者(英) | ||||||
en | ||||||
Tamai, Takayuki | ||||||
著者(英) | ||||||
en | ||||||
Kuwabara, Yasuhiro | ||||||
著者(英) | ||||||
en | ||||||
Ouchi, Seiji | ||||||
著者 所属 | ||||||
近畿大学農学総合研究所 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学薬学総合研究所 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
版 | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 名前 | ||||||
出版者 | 近畿大学農学総合研究所 | |||||
書誌情報 |
近畿大学農学総合研究所報告 en : Bulletin of the Institute for Comprehensive Agricultural Sciences, Kinki University 号 9, p. 129-140, 発行日 2001-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09193022 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | [Synopsis] In this study, we cloned a chitinase gene from Burkholderia cepacia and identified a nucleotide sequence. A genomic library was constructed by the use of λEMBL3, and then phage clones producing chitinase were selected by using 4-MU chitotrioside as a substrate. The inserted DNA was subcloned into a pBluescript vector with restriction enzymes to make pBlueCAK12 (4.4 kb) clone including a chitinase gene. The DNA fragment was deleted by exonuclease III and the chitinase-coding region was determined by chitinase activity detected by the use of 4-MU chitotrioside. The nucleotide sequence of chitinase gene (2.98 kb) was determined by primer walking method, in which ORF of chitinase gene was amplified by specific primers including the expected start and stop codons. The amplified DNA was inserted into a pBluescript vector, and then chitinase activity was detected by the use of 4-MU chitotrioside. | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |