{"created":"2023-06-20T16:39:42.521673+00:00","id":9845,"links":{},"metadata":{"_buckets":{"deposit":"1d887b1b-1dec-498e-a9e7-1d0b6a44933f"},"_deposit":{"created_by":3,"id":"9845","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9845"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00009845","sets":["14:923:2473:2474","21:3039:3413:3419"]},"author_link":["17817","17819","17815","17813","17816","17818","17812","17814"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"DNA sequence of chitinase gene cloned from Burkholderia cepacia"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"140","bibliographicPageStart":"129","bibliographic_titles":[{"bibliographic_title":"近畿大学農学総合研究所報告"},{"bibliographic_title":"Bulletin of the Institute for Comprehensive Agricultural Sciences, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[Synopsis] In this study, we cloned a chitinase gene from Burkholderia cepacia and identified a nucleotide sequence. A genomic library was constructed by the use of λEMBL3, and then phage clones producing chitinase were selected by using 4-MU chitotrioside as a substrate. The inserted DNA was subcloned into a pBluescript vector with restriction enzymes to make pBlueCAK12 (4.4 kb) clone including a chitinase gene. The DNA fragment was deleted by exonuclease III and the chitinase-coding region was determined by chitinase activity detected by the use of 4-MU chitotrioside. The nucleotide sequence of chitinase gene (2.98 kb) was determined by primer walking method, in which ORF of chitinase gene was amplified by specific primers including the expected start and stop codons. The amplified DNA was inserted into a pBluescript vector, and then chitinase activity was detected by the use of 4-MU chitotrioside.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学農学総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09193022","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Matsuda, Yoshinori"},{"subitem_text_language":"en","subitem_text_value":"Toyoda, Hideyoshi"},{"subitem_text_language":"en","subitem_text_value":"Nonomura, Teruo"},{"subitem_text_language":"en","subitem_text_value":"Kakutani, Koji"},{"subitem_text_language":"en","subitem_text_value":"Ikeda, Seishi"},{"subitem_text_language":"en","subitem_text_value":"Tamai, Takayuki"},{"subitem_text_language":"en","subitem_text_value":"Kuwabara, Yasuhiro"},{"subitem_text_language":"en","subitem_text_value":"Ouchi, Seiji"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学総合研究所"},{"subitem_text_value":"近畿大学農学部農学科"},{"subitem_text_value":"近畿大学農学部農学科"},{"subitem_text_value":"近畿大学薬学総合研究所"},{"subitem_text_value":"近畿大学農学部農学科"},{"subitem_text_value":"近畿大学農学部農学科"},{"subitem_text_value":"近畿大学農学部農学科"},{"subitem_text_value":"近畿大学農学部農学科"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松田,  克礼"},{"creatorName":"マツダ, ヨシノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"豊田,  秀吉"},{"creatorName":"トヨダ, ヒデヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"野々村,  照雄"},{"creatorName":"ノノムラ, テルオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"角谷,  晃司"},{"creatorName":"カクタニ, コウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"池田,  成志"},{"creatorName":"イケダ, セイシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"玉井,  隆行"},{"creatorName":"タマイ, タカユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"桑原,  精宏"},{"creatorName":"クワバラ, ヤスヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大内,  成志"},{"creatorName":"オオウチ, セイジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AN10410378-20010315-0129.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN10410378-20010315-0129.pdf","url":"https://kindai.repo.nii.ac.jp/record/9845/files/AN10410378-20010315-0129.pdf"},"version_id":"ba12ba2e-fe1b-4289-9cf1-66b2b32c749f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Burkholderia cepaciaからクローニングしたキチナーゼ遺伝子の塩基配列決定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Burkholderia cepaciaからクローニングしたキチナーゼ遺伝子の塩基配列決定"}]},"item_type_id":"2","owner":"3","path":["2474","3419"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-11-21"},"publish_date":"2011-11-21","publish_status":"0","recid":"9845","relation_version_is_last":true,"title":["Burkholderia cepaciaからクローニングしたキチナーゼ遺伝子の塩基配列決定"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:52:38.170265+00:00"}