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SUBFUNCTIONALIZATION OF ENDONUCLEASE G ISOZYMES IN XENOPUS TROPICALIS
https://doi.org/10.15100/0002000990
https://doi.org/10.15100/000200099095b7b9ee-293b-4154-8bf3-bd6837f2d606
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA11574630-20240331-0025.pdf
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| Item type | 紀要論文 / departmental bulletin paper(1) | |||||||||
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| 公開日 | 2024-03-27 | |||||||||
| タイトル | ||||||||||
| タイトル | SUBFUNCTIONALIZATION OF ENDONUCLEASE G ISOZYMES IN XENOPUS TROPICALIS | |||||||||
| 言語 | en | |||||||||
| 作成者 |
Sou, Natsuki
× Sou, Natsuki
× Tokmakov, Alexander A.
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | endonuclease G, homology modeling, predictive bioinformatics, Xenopus tropicalis | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Endonuclease G (EndoG) is known to be involved in apoptotic degradation of nuclear DNA. It has also been proposed to carry out mRNA degradation in the cytoplasm of apoptotic cells. In the present work, predictive bioinformatics analysis and comparative homology modeling were used to reveal subfunctionalization of two EndoG isozymes (xtEndoG) in the Xenopus tropicalis western clawed frog. The analysis suggests that mitochondrial translocation of one of these proteins can be regulated by calcium-dependent phosphorylation in the region of its N-terminal signal sequence. Detailed comparison of modeled 3D structures of the two proteins reveals thermodynamic and structural differences that affect local protein stability. Our findings bring to light a novel mechanism that can possibly regulate mitochondrial translocation of EndoG and provide a rationale for the existence of two highly homologous xtEndoG proteins in Xenopus tropicalis. Here, we also discuss a probable involvement of the calcium-dependent mechanism of EndoG translocation in frog egg apoptosis. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 出版者 | 近畿大学先端技術総合研究所 | |||||||||
| 言語 | ja | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.15100/0002000990 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 13468693 | |||||||||
| 書誌情報 |
ja : 近畿大学先端技術総合研究所紀要 en : Memoirs of Institute of Advanced Technology, Kindai University 号 29, p. 25-33, 発行日 2024-03-31 |
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