| Item type |
☆紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2018-07-26 |
| タイトル |
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タイトル |
<Original> Atorvastatin promoted in vitro angiogenesis by reduction of geranylgeranyl pyrophosphate in a dose-dependent manner and protected against rho kinase-mediated endothelial cell damage caused by thromboxane A2 |
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言語 |
en |
| 著者 |
Yokota, Ryoji
Nishi, Yuko
Sugitani, Yuuki
Tamada, Hiroyuki
Ohi, Yohei
Ishikawa, Chisato
Ohe, Kentarou
Uemori, Noritsugu
Mitsuoka, Hirokazu
Togi, Kiyonori
Shirotani, Manabu
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| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
Angiogenesis, Endothelial cells, Statin, Geranylgeranyl pyrophosphate, Rho kinase, Thromboxane A2, Caspase-3 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 著者 所属 |
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値 |
Cardiovascular Center, Nara Hospital, Kindai University Faculty of Medicine |
| 版 |
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出版タイプ |
NA |
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出版タイプResource |
http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| 出版者 名前 |
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出版者 |
Kindai University Medical Association |
| 書誌情報 |
en : ACTA MEDICA KINDAI UNIVERSITY
巻 43,
号 1,
p. 35-42,
発行日 2018-06
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03866092 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
[Abstract] Background: Atorvastatin can inactivate Rho/Rho kinase via a reduction in the synthesis of geranylgeranyl pyrophosphate (GGPP).Thromboxane A2 (TxA2) causes endothelial cell (EC) apoptosis via Rho/Rho kinase activation.We tested the hypothesis that atorvastatin protects against the Rho kinase-mediated anti-angiogenic effect of TxA2. Methods: We used human coronary artery ECs to form tubular structures on plates coated with a basement membrane matrix gel. The number of tubular structure was counted under a microscope. The caspase-3 activity was used as a determinant of apoptosis.Results: Atorvastatin significantly increased the number of tubes in a dose-dependent manner, and this effect was blocked by mevalonate or geranylgeranyl pyrophosphate (GGPP). Similar to atorvastatin, a potent selective inhibitor of geranylgeranyl transferase type I enhanced tubular formation. A TxA2 mimetic (IBOP) inhibited formation of EC tubular structures. The inhibitory effect was completely blocked by a TxA2 antagonist (SQ29548), a Rho kinase inhibitor (Y27632), and by atorvastatin. The IBOP-induced increase in caspase-3 activity was attenuated by atorvastatin. Conclusions: Atorvastatin promoted in vitro angiogenesis of ECs in a dose-dependent manner and reversed the TxA2 receptor-mediated antiangiogenic effect. We suggest that reduction of GGPP and inactivation of Rho kinase plays an important role in the proangiogenic effect of atorvastatin. |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
AA0050842X-20180600-0035.pdf (713.7 kB)
