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IVM culture in mTaM containing 1-10 mM L-carnitine showed well maturation rate ranged from 82% to 98%. Then, MII oocytes normally developed in mTaM containing L-carnitine were measured the yield of ROS in ooplasm by oxidized DCFDA detection assay with a fluorescence microscope. As a result, fluorescence intensity of L-carnitine-treated groups ranged from 353.61 to 415.62 pixels in comparison to 581.82 pixels in L-carnitine-free control experiment. Furthermore, in vitro matured oocytes treated with L-carnitine showed lower fluorescence intensity than ovulated oocytes. 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〈研究ノート〉L -カルニチン添加体外成熟培地がマウス卵子細胞質内活性酸素種に与える影響
https://kindai.repo.nii.ac.jp/records/17648
https://kindai.repo.nii.ac.jp/records/176480130ec84-3f51-4a47-93b6-2bb906a2c0c1
名前 / ファイル | ライセンス | アクション |
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AA11574630-20160531-0049.pdf (654.1 kB)
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Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-08-02 | |||||
タイトル | ||||||
タイトル | 〈研究ノート〉L -カルニチン添加体外成熟培地がマウス卵子細胞質内活性酸素種に与える影響 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effect of in vitro maturation medium containing L-carnitine on reactive oxygen species in immature oocytes | |||||
著者 |
井上, 達也
× 井上, 達也× 東, 里香× 野田, 義博× 西村, 愛美× 梶本, みずき× 小橋, 朱里× 折杉, 卓哉× 細井, 美彦× 安齋, 政幸 |
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言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | マウス, 未成熟卵子, 体外成熟, L - カルニチン, 活性酸素種 mouse, immature oocytes, in vitro maturation, L-carnitine, reactive oxygen species |
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資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者(英) | ||||||
en | ||||||
Inoue, Tatsuya | ||||||
著者(英) | ||||||
en | ||||||
Azuma, Rika | ||||||
著者(英) | ||||||
en | ||||||
Noda, Yoshihiro | ||||||
著者(英) | ||||||
en | ||||||
Nishimura, Manami | ||||||
著者(英) | ||||||
en | ||||||
Kajimoto, Mizuki | ||||||
著者(英) | ||||||
en | ||||||
Obashi, Akari | ||||||
著者(英) | ||||||
en | ||||||
Orisugi, Takuya | ||||||
著者(英) | ||||||
en | ||||||
Hosoi, Yoshihiko | ||||||
著者(英) | ||||||
en | ||||||
Anzai, Masayuki | ||||||
著者 所属 | ||||||
近畿大学生物理工学部遺伝子工学科 | ||||||
著者 所属 | ||||||
近畿大学大学院生物理工学研究科生物工学専攻 | ||||||
著者 所属 | ||||||
(地独)東京都健康長寿医療センター研究所 | ||||||
著者 所属 | ||||||
関西医科大学付属生命研究所 | ||||||
著者 所属 | ||||||
近畿大学生物理工学部遺伝子工学科 | ||||||
著者 所属 | ||||||
近畿大学生物理工学部遺伝子工学科 | ||||||
著者 所属 | ||||||
近畿大学生物理工学部遺伝子工学科 | ||||||
著者 所属 | ||||||
近畿大学生物理工学部遺伝子工学科:近畿大学大学院生物理工学研究科生物工学専攻:近畿大学先端技術総合研究所 | ||||||
著者 所属 | ||||||
近畿大学先端技術総合研究所 | ||||||
著者所属(翻訳) | ||||||
Kindai University | ||||||
著者所属(翻訳) | ||||||
Kindai University | ||||||
著者所属(翻訳) | ||||||
Tokyo Metropolitan Institute of Gerontology | ||||||
著者所属(翻訳) | ||||||
Kansai Medical University | ||||||
著者所属(翻訳) | ||||||
Kindai University | ||||||
著者所属(翻訳) | ||||||
Kindai University | ||||||
著者所属(翻訳) | ||||||
Kindai University | ||||||
著者所属(翻訳) | ||||||
Kindai University | ||||||
著者所属(翻訳) | ||||||
Kindai University | ||||||
版 | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 名前 | ||||||
出版者 | 近畿大学先端技術総合研究所 | |||||
書誌情報 |
近畿大学先端技術総合研究所紀要 en : Memoirs of Institute of Advanced Technology, Kindai University 巻 21, p. 49-56, 発行日 2016-05-31 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13468693 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 本研究では、私たちが以前報告した、修正TaM 培地へL -カルニチンを添加した体外成熟培地を用いたマウス未成熟卵子への体外成熟培養をおこない、卵子細胞質内で産生される活性酸素種に与える影響を検討した。修正TaM 培地へL -カルニチンをそれぞれ1mM, 2mM, 5mM, 10mM 添加し体外成熟培養をおこなった結果、82~98%が正常なMⅡ期卵子へと発生した。次に、各L -カルニチン添加区において、正常に発生した体外成熟卵子は、卵子細胞質内における活性酸素種の産生を蛍光輝度より測定した。その結果、活性酸素種の産生は、それぞれ353.61 ± 15.34 ~ 415.62 ± 25.82 pixel の蛍光強度を発しており、全てのL -カルニチン添加区は、非添加区(581.82±43.33 pixel)と比較し過剰な活性酸素種の産生を抑制することが示された。 In this study, we investigated the effect of L-carnitine in IVM medium( modified TaM; mTaM) on the production of reactive oxygen species( ROS) in ooplasm during in vitro maturation( IVM) of immature oocytes. IVM culture in mTaM containing 1-10 mM L-carnitine showed well maturation rate ranged from 82% to 98%. Then, MII oocytes normally developed in mTaM containing L-carnitine were measured the yield of ROS in ooplasm by oxidized DCFDA detection assay with a fluorescence microscope. As a result, fluorescence intensity of L-carnitine-treated groups ranged from 353.61 to 415.62 pixels in comparison to 581.82 pixels in L-carnitine-free control experiment. Furthermore, in vitro matured oocytes treated with L-carnitine showed lower fluorescence intensity than ovulated oocytes. In conclusion, it can be considered that L-carnitine in IVM medium reduces an excessive yield of ROS in ooplasm in in vitro maturation of immature oocytes. |
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フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |