{"created":"2023-06-20T16:39:51.623076+00:00","id":9996,"links":{},"metadata":{"_buckets":{"deposit":"ea7958d7-3470-4ba4-9a3b-ddce8aa3004a"},"_deposit":{"created_by":3,"id":"9996","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9996"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00009996","sets":["14:923:2219:2231","21:3039:3435:3436"]},"author_link":["18266","18273","18269","18268","18270","18271","18272","18267"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Investigation of zona drilled by laser and in vitro fertilization of oocytes that matured in vitro with C57BL/6 strain"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageEnd":"35","bibliographicPageStart":"27","bibliographic_titles":[{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University"},{"bibliographic_title":"近畿大学先端技術総合研究所紀要","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本実験では成熟齢C57BL/6J マウスから得られた体外成熟卵子を作出し、レーザー穿孔処理法を用いて各出力条件下（200, 150, 120μsec.）で透明帯穿孔処理を行ない、その後の体外受精および発生能を検討した。C57BL/6J 卵巣より回収した未成熟卵子の体外成熟成績は、91％（1, 517/1, 674）であった。レーザー穿孔処理時間による受精成績は、それぞれ、60％（191/316）, 54％（103/192）, 45％（196/439）であり、対照区（28％：129/463）と比較し有意な差が認められた（P<0.05）。また一部を培養した結果、胚盤胞期への発生率は31％（32/102）, 51％（74/144）, 53％（40/75）であった。２細胞期胚を移植した結果、レーザー出力を低出力にした場合、産子の発生向上が確認された〔6％（5/81）, 13％（10/83）, 21％（12/56）〕。さらにレーザー照射による熱変性を避けるため、卵細胞質を収縮させた透明帯穿孔卵子における受精成績も同様に対照区と比較し有意に向上した（p<0.05）。以上の結果より、C57BL/6J 未成熟卵子の体外成熟およびレーザー穿孔処理の条件を調整することにより、産子への発生を改善することが示唆された。","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Nishimura, Manami"},{"subitem_text_language":"en","subitem_text_value":"Omoto, Nami"},{"subitem_text_language":"en","subitem_text_value":"Nishiyama, Yui"},{"subitem_text_language":"en","subitem_text_value":"Yanagi, Miho"},{"subitem_text_language":"en","subitem_text_value":"Mitani, Tasuku"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"},{"subitem_text_language":"en","subitem_text_value":"Iritani, Akira"},{"subitem_text_language":"en","subitem_text_value":"Anzai, Masayuki"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究"},{"subitem_text_value":"アーク・リソース株式会社"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"ARK Resource Co., Ltd."},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西村, 　愛美"},{"creatorName":"ニシムラ, マナミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大本, 　夏未"},{"creatorName":"オオモト, ナミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西山, 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