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Efficient Use of the Green Fluorescence Protein Gene for Genetic Marking of Fusarium oxysporum f. sp. spinaciae
https://kindai.repo.nii.ac.jp/records/9899
https://kindai.repo.nii.ac.jp/records/9899c9e6697f-b556-402c-bd34-3ab6dbf447c7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN10410378-19980315-0053.pdf (393.4 kB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||||||
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| 公開日 | 2011-11-25 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Efficient Use of the Green Fluorescence Protein Gene for Genetic Marking of Fusarium oxysporum f. sp. spinaciae | |||||||||||||||||||||
| 著者 |
Nonomura, Teruo
× Nonomura, Teruo
× Toyoda, Hideyoshi
× Matsuda, Yoshinori
× Ouchi, Seiji
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| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||||||
| 著者 所属 | ||||||||||||||||||||||
| 値 | Faculty of Agriculture, Kinki University | |||||||||||||||||||||
| 著者 所属 | ||||||||||||||||||||||
| 値 | Faculty of Agriculture, Kinki University | |||||||||||||||||||||
| 著者 所属 | ||||||||||||||||||||||
| 値 | Institute for Comprehensive Agricultural Sciences, Kinki University | |||||||||||||||||||||
| 著者 所属 | ||||||||||||||||||||||
| 値 | Faculty of Agriculture and Institute for Comprehensive Agricultural Sciences, Kinki University | |||||||||||||||||||||
| 版 | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| 出版者 名前 | ||||||||||||||||||||||
| 出版者 | 近畿大学農学総合研究所 | |||||||||||||||||||||
| 書誌情報 |
近畿大学農学総合研究所報告 en : Bulletin of the Institute for Comprehensive Agricultural Sciences, Kinki University 号 6, p. 53-56, 発行日 1998-03-01 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 09193022 | |||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | [Synopsis] To facilitate monitoring the infection behaviour of Fusarium oxysporum in planta, the hygromycinresistant gene and the green fluorescent protein gene were introduced into microconidia of F.oxysporum f. sp. spinaciae. The microconidia were subjected to high voltage pulse in the prescence of the plasmid for electroporation and the hygromycin-resistant transformants producing the green fluorescent protein were selected under UV irradiation. The integration of the marker genes into chromosomal DNA of these transformants was confiremed by polymerase chain reaction and Southern hybridization analysis. Transformation of phytopathogenic fungus with the green fluorescence gene enable us to easily and effectively detect the gene-marked fungi under UV-light without any additional chromogenic substrates for detecting translation product. | |||||||||||||||||||||
| フォーマット | ||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||||||
