| Item type |
☆紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2011-11-21 |
| タイトル |
|
|
タイトル |
Circulated Culture System of Tobacco Hairy Roots for Rapid Evaluation of Viral Multiplication and Movement |
| 著者 |
Matsuda, Yoshinori
Toyoda, Hideyoshi
Konagaya, Kenichi
Wakita, Makoto
Hosoi, Yoshiyuki
Nonomura, Teruo
Ouchi, Seiji
|
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 所属 |
|
|
値 |
Division of Bioengineering, Institute for Comprehensive Agricultural Sciences, Kinki University |
| 著者 所属 |
|
|
値 |
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University |
| 著者 所属 |
|
|
値 |
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University |
| 著者 所属 |
|
|
値 |
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University |
| 著者 所属 |
|
|
値 |
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University |
| 著者 所属 |
|
|
値 |
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University |
| 著者 所属 |
|
|
値 |
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University |
| 版 |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 名前 |
|
|
出版者 |
近畿大学農学総合研究所 |
| 書誌情報 |
近畿大学農学総合研究所報告
en : Bulletin of the Institute for Comprehensive Agricultural Sciences, Kinki University
号 9,
p. 81-88,
発行日 2001-03-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09193022 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
[Synopsis] From the hairy roots induced by an inoculation of Agrobacterium rhizogenes into tobacco (Nicotiana tabacum cv. Bright Yellow) leaf segments, we obtained the hairy root clone which could frequently regenerate shoots from calli produced at the basal sites of lateral roots branched from major hairy roots. The leaflets detached from the regenerated shoots produced secondarily the hairy roots and differentiated into the secondary shoots via calli in a similar manner. This circulatory culture system for the hairy root production and plant regeneration was available for an in vitro assay of multiplication and translocation of TMV. By the inoculation into detached leaves of the regenerated shoots with TMV, the virus-specific mosaic symptom was observed in the leaflets of the newly regenerated shoots, and the localization of TMV in the symptomless tissues such as stems, petioles, and secondary hairy roots of the infected regenerants was easily detected by direct tissue printing immunoblotting assay. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Konagaya, Ken-ichi |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
AN10410378-20010315-0081.pdf (820.7 kB)
