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Molecular cloning and structural analyses of the pyrF gene for orotate monophosphate (OMP)decarboxylase from extremely halophilic archaeon Haloarcula japonica strain TR-1
https://kindai.repo.nii.ac.jp/records/7280
https://kindai.repo.nii.ac.jp/records/72807ab33cde-fe5c-460e-b290-deeaee44e253
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063799-20081220-0013.pdf (795.0 kB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||
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| 公開日 | 2010-07-02 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Molecular cloning and structural analyses of the pyrF gene for orotate monophosphate (OMP)decarboxylase from extremely halophilic archaeon Haloarcula japonica strain TR-1 | |||||||||||||
| 著者 |
Maeda, Yutaka
× Maeda, Yutaka
× Nakasone, Kaoru
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| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | Haloarcula japonica, pyfF, pyrimidine biosynthesis, 5-FOA, auxotroph | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||
| 著者(英) | ||||||||||||||
| 言語 | en | |||||||||||||
| 値 | 前田, 豊 | |||||||||||||
| 著者(英) | ||||||||||||||
| 言語 | en | |||||||||||||
| 値 | 仲宗根, 薫 | |||||||||||||
| 著者 所属 | ||||||||||||||
| 値 | 近畿大学大学院システム工学研究科 | |||||||||||||
| 著者 所属 | ||||||||||||||
| 値 | 近畿大学大学院システム工学研究科 | |||||||||||||
| 著者所属(翻訳) | ||||||||||||||
| 値 | Graduate School of Systems Engineering Kinki, University | |||||||||||||
| 著者所属(翻訳) | ||||||||||||||
| 値 | Graduate School of Systems Engineering Kinki, University | |||||||||||||
| 版 | ||||||||||||||
| 出版タイプ | NA | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||||||
| 出版者 名前 | ||||||||||||||
| 出版者 | 近畿大学工学部 | |||||||||||||
| 書誌情報 |
近畿大学工学部研究報告 en : Research reports of the School of Engineering, Kinki University 号 42, p. 13-16, 発行日 2008-12-01 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0386491X | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The orotate monophosphate decarboxylase gene (pyrF) in the pyrimidine biosynthetic pathway have unique characteristics. In this study, the pyrF gene was found to encode a polypetide consisting of 277 amino acid residues, showing 96,0 ％ identity to that of H.marismortui. Southern hybridization of the pyfF gene region with partial sequence probe revealed that single copy number of the gene is distributed on the chromosome of H. japonica genome. By phylogenetic analyses of the H. japonica PyrF, the amino acid sequences of the enzyme is relatively conserved in other archaeal strains including methanogens and thermophiles. Alignments of several sequences containing pyrF gene showed that the genetic organization of pyrF gene was not conserved in several haloarchaeal strains expect for H.marismortui. | |||||||||||||
| フォーマット | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | application/pdf | |||||||||||||
