{"created":"2023-06-20T16:36:16.615428+00:00","id":5772,"links":{},"metadata":{"_buckets":{"deposit":"7006bb55-aa5a-49e8-b9ce-5c778992e61d"},"_deposit":{"created_by":3,"id":"5772","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5772"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00005772","sets":["14:923:1549:1559","21:3039:3109:3141"]},"author_link":["8824","8825","8827","8826","8829","8830","8828"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"〈Original Papers〉タバコモザイクウイルス外被蛋白質の大腸菌での大量生産ならびに自己会合の解析"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-09-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"57","bibliographicPageStart":"51","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University"},{"bibliographic_title":"近畿大学生物理工学部紀要","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[Abstract] Tobacco mosaic virus coat protein is known to self-associate into several kinds of supramolecular structures, and presents a model system suitable for protein-engineering of supramolecular assembly. Here, as a step towards such studies, we have developed an auto-inducing expression system of the coat protein in E.coli and a simple protein purification method under acidic solution conditions. As much as 114 mg of nearly homogeneous protein per three liter of culture was obtained. The yield is much higher than that obtained by using a conventional IPTG-induction method. Size-exclusion chromatography has shown that the protein exists in three states of assembly at pH 7.2, 150mM NaCl: high Mw, medium Mw (2000 k — 600 k), and low Mw (＜40 k). Atomic force microscopic observation showed that assemblages of 20 nm in diameter, 4.5 nm in height, and with a central hole, which resemble the \"disk\" structure of wild-type coat protein, existed in the medium Mw fraction. Altogether, this system is expected to be useful in large-scale production of variant recombinant coat proteins for the protein-engineering study of supramolecular assembly.           [要旨] タバコモザイクウイルス外被蛋白質はいろいろな超分子構造に自己集合することが知られており、超分子集合の蛋白質工学研究のモデル系として好適である。ここでは、そのような研究への一歩として、タバコモザイクウイルス外被蛋白質の大腸菌での「自動誘導法」による生産、ならびに酸性溶液条件下における簡便な蛋白質精製について報告する。培養液3Lあたり114mgという、従来のIPTG誘導法に比べてずっと多量の、ほぼ均一な外被蛋白質が得られた。サイズ排除クロマトグラフィ法により、pH7.2、150mM NaCl存在下でこの蛋白質は三つの会合状態をとることが示された。すなわち、高分子量、中間分子量(2000k-600k)低分子量(＜40k)の3分画である。原子間力顕微鏡下では、中間分子量分画に、直径20nm、高さ4.5nmで中央に穴の開いた、天然のタバコモザイクウイルス外被蛋白質で知られているディスク構造に似た集合体が観察された。以上、この系は、超分子自己集合体の蛋白質工学研究のために人工的に変異を導入した組換え蛋白質を大量に調製するのに役立つと思われる。","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"【注記】This study was partially supported by the Project Research of the Faculty of Biology-Oriented Science and Technology No.08-II-1,2009-2011","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学生物理工学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13427202","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"田中, 宏幸"},{"subitem_text_language":"en","subitem_text_value":"河野, 良平"},{"subitem_text_language":"en","subitem_text_value":"石橋, 和大"},{"subitem_text_language":"en","subitem_text_value":"石川, 雅之"},{"subitem_text_language":"en","subitem_text_value":"飯, 哲夫"},{"subitem_text_language":"en","subitem_text_value":"米澤, 康滋"},{"subitem_text_language":"en","subitem_text_value":"橘, 秀樹"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University:High Pressure Protein Research Center, Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba"},{"subitem_text_value":"Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba"},{"subitem_text_value":"Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba"},{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University:High Pressure Protein Research Center, Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University:High Pressure Protein Research Center, Institute of Advanced Technology, Kinki University"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"和歌山県立医科大学機能性医薬食品探索講座:近畿大学先端技術総合研究所高圧力蛋白質研究センター"},{"subitem_text_value":"農業生物資源研究所植物科学研究領域"},{"subitem_text_value":"農業生物資源研究所植物科学研究領域"},{"subitem_text_value":"農業生物資源研究所植物科学研究領域"},{"subitem_text_value":"近畿大学大学院生物理工学研究科:近畿大学先端技術総合研究所高圧力蛋白質研究センター"},{"subitem_text_value":"近畿大学大学院生物理工学研究科:近畿大学先端技術総合研究所高圧力蛋白質研究センター"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanaka,  Hiroyuki"},{"creatorName":"タナカ, ヒロユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono,  Ryohei"},{"creatorName":"コウノ, リョウヘイ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8825","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishibashi,  Kazuhiro"},{"creatorName":"イシバシ, カズヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8826","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa,  Masayuki"},{"creatorName":"イシカワ, マサユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Meshi,  Tetsuo"},{"creatorName":"メシ, テツオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8828","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yonezawa,  Yasushige"},{"creatorName":"ヨネザワ, ヤスシゲ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tachibana,  Hideki"},{"creatorName":"タチバナ, ヒデキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8830","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11153712-20130930-0051.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11153712-20130930-0051.pdf","url":"https://kindai.repo.nii.ac.jp/record/5772/files/AA11153712-20130930-0051.pdf"},"version_id":"915e5f18-81e4-4c66-8237-5a8587ba9c56"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"タバコモザイクウイルス","subitem_subject_scheme":"Other"},{"subitem_subject":"外被蛋白質","subitem_subject_scheme":"Other"},{"subitem_subject":"超分子集合体","subitem_subject_scheme":"Other"},{"subitem_subject":"蛋白質工学","subitem_subject_scheme":"Other"},{"subitem_subject":"tobacco mosaic virus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"coat protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"supramolecular assembly","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein engineering","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈Original Papers〉Large-Scale Production of Tobacco Mosaic Virus Coat Protein in E.coli and Analysis of its Self-Association","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈Original Papers〉Large-Scale Production of Tobacco Mosaic Virus Coat Protein in E.coli and Analysis of its Self-Association","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["1559","3141"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-11-16"},"publish_date":"2013-11-16","publish_status":"0","recid":"5772","relation_version_is_last":true,"title":["〈Original Papers〉Large-Scale Production of Tobacco Mosaic Virus Coat Protein in E.coli and Analysis of its Self-Association"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T19:20:03.607684+00:00"}