@article{oai:kindai.repo.nii.ac.jp:00004973,
 author = {光永,  俊郎 and 安藤,  ひとみ},
 issue = {23},
 journal = {近畿大学農学部紀要, Memoirs of the Faculty of Agriculture of Kinki University},
 month = {Jan},
 note = {[Author abstract]In the biuret determination of protein in rices and rice products, starch and lipids extractable with an alkaline solution such as biuret reagent were found to interfere with the biuret method. Comparative evaluation of their effects revealed that starch, a major component of rice, has the most significant effect on the biuret method. In the presence of starch, the correlation between Kjeldahl protein and the optical density of biuret was poor. It was found that several perhalocarbon solvents effectively retarded solubilization of rice starch in the biuret reaction mixture. By treatment of the rice samples with these solvents prior to the biuret procedure, the effect of starch was eliminated and the optical density of biuret became closely correlated with Kjeldahl protein.[著者抄録]米および米製品のタンパク質の簡易定量法としてビュレット法を検討した. 1.米試料を直接ビュレット試薬で抽出,反応する方法では, Kjeldahlタンパク質とビュレット吸光度の相関係数は0.815であった. 2.米試料のアルカリ抽出液についてビュレット法の妨害因子を調べた結果,デンプンが顕著な妨害効果を示した.3.デンプンの防害因子抑制には炭素数3～6のパーハロカーボンが効果的であった. 4.米試料をパーハロカーボンで前処理して,ビュレット法でタンパク質を定量すると,その吸光度と各試料とのKjeldahlタンパク質との間には,0.944以上の高い相関係数が認められた., 記事区分：原著, application/pdf},
 pages = {63--66},
 title = {米および米製品タンパク質の簡易定量法について},
 year = {1990},
 yomi = {ミツナガ, トシオ and アンドウ, ヒトミ}
}