{"created":"2023-06-20T16:35:29.099124+00:00","id":4820,"links":{},"metadata":{"_buckets":{"deposit":"daadd4f7-a44e-4e2e-8679-5dc12db47f40"},"_deposit":{"created_by":3,"id":"4820","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4820"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00004820","sets":["14:923:1379:1405","21:3039:3040:3045"]},"author_link":["6494","6497","6495","6496"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Simultaneous detection of GFP- and GUS-marked fungi of different formae speciales of Fusarium oxysporum on plant roots"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"36","bibliographicPageEnd":"26","bibliographicPageStart":"21","bibliographic_titles":[{"bibliographic_title":"近畿大学農学部紀要"},{"bibliographic_title":"Memoirs of the Faculty of Agriculture of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In our attempt  to visualize the infection behavior of fungal wilt pathogens inoculated onto plant roots, the fungi were genetically marked with two reporter genes. Fusarium oxysporum f. sp. Melonis (FOM) and F. o. f. sp. Lycopersici (FOL) were transformed using the green fluorescence protein gene (GFP) and the β-glucuronidase gene (GUS), respectively. In the present study, we attempted to directly distinguish different formae speciales of F. oxysporum on the same plant roots by expression of different marker genes. Microconidia of GFP-marked FOM (FOM-001) and GUS-marked FOL (FOL-005) were inoculated onto roots of cotyledonal seedings, and inoculated roots were first observed under a fluorescence microscope in order to detect FOM-001 and then stained with X-gluc (substrate for GUS assay) in order to detect FOL-005 under a light microscope. Consequently, both transformed pathogens could be clearly  distinguished at the same site of inoculation. These results suggest that the dual transformation of F. oxysporum is useful for analyzing the behavior of nonpathogenic F. oxysporum challenge-inoculated with pathogenic F. oxysporum.","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"記事区分：原著","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学農学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04538889","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Nonomura, Teruo"},{"subitem_text_language":"en","subitem_text_value":"Hatasa, Tetsuya"},{"subitem_text_language":"en","subitem_text_value":"Maeda, Kazuhiko"},{"subitem_text_language":"en","subitem_text_value":"Toyoda, Hideyoshi"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部農学科"},{"subitem_text_value":"近畿大学農学部農学科"},{"subitem_text_value":"近畿大学湯浅農場"},{"subitem_text_value":"近畿大学農学部農学科"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Department of Agriculture, Faculty of Agriculture, Kinki University"},{"subitem_text_value":"Department of Agriculture, Faculty of Agriculture, Kinki University"},{"subitem_text_value":"Experimental Farm, Kinki University"},{"subitem_text_value":"Department of Agriculture, Faculty of Agriculture, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野々村,  照雄"},{"creatorName":"ノノムラ, テルオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"畑佐,  哲也"},{"creatorName":"ハタサ, テツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"前田,  和彦"},{"creatorName":"マエダ, カズヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"豊田,  秀吉"},{"creatorName":"トヨダ, ヒデヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AN00064044-20030331-0021.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00064044-20030331-0021.pdf","url":"https://kindai.repo.nii.ac.jp/record/4820/files/AN00064044-20030331-0021.pdf"},"version_id":"47344ae6-cfe9-4129-8613-8d58b5b721eb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_thumbnail":{"attribute_name":"サムネイル","attribute_value_mlt":[{"subitem_thumbnail":[{"thumbnail_label":"AN00064044-20030331-0021.jpeg","thumbnail_url":"/api/files/daadd4f7-a44e-4e2e-8679-5dc12db47f40/AN00064044-20030331-0021.jpeg?versionId=6e276efe-89e3-4411-a94a-7f20e4b44ad8"}]}]},"item_title":"トマト根に同時接種したフザリウム病菌の遺伝子標識法による識別","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"トマト根に同時接種したフザリウム病菌の遺伝子標識法による識別"}]},"item_type_id":"2","owner":"3","path":["1405","3045"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-01-28"},"publish_date":"2008-01-28","publish_status":"0","recid":"4820","relation_version_is_last":true,"title":["トマト根に同時接種したフザリウム病菌の遺伝子標識法による識別"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T00:58:27.545561+00:00"}