@article{oai:kindai.repo.nii.ac.jp:00004820,
 author = {野々村,  照雄 and 畑佐,  哲也 and 前田,  和彦 and 豊田,  秀吉},
 issue = {36},
 journal = {近畿大学農学部紀要, Memoirs of the Faculty of Agriculture of Kinki University},
 month = {Jan},
 note = {In our attempt  to visualize the infection behavior of fungal wilt pathogens inoculated onto plant roots, the fungi were genetically marked with two reporter genes. Fusarium oxysporum f. sp. Melonis (FOM) and F. o. f. sp. Lycopersici (FOL) were transformed using the green fluorescence protein gene (GFP) and the β-glucuronidase gene (GUS), respectively. In the present study, we attempted to directly distinguish different formae speciales of F. oxysporum on the same plant roots by expression of different marker genes. Microconidia of GFP-marked FOM (FOM-001) and GUS-marked FOL (FOL-005) were inoculated onto roots of cotyledonal seedings, and inoculated roots were first observed under a fluorescence microscope in order to detect FOM-001 and then stained with X-gluc (substrate for GUS assay) in order to detect FOL-005 under a light microscope. Consequently, both transformed pathogens could be clearly  distinguished at the same site of inoculation. These results suggest that the dual transformation of F. oxysporum is useful for analyzing the behavior of nonpathogenic F. oxysporum challenge-inoculated with pathogenic F. oxysporum., 記事区分：原著, application/pdf},
 pages = {21--26},
 title = {トマト根に同時接種したフザリウム病菌の遺伝子標識法による識別},
 year = {2003},
 yomi = {ノノムラ, テルオ and ハタサ, テツヤ and マエダ, カズヒコ and トヨダ, ヒデヨシ}
}