{"created":"2023-06-20T16:34:23.039488+00:00","id":3563,"links":{},"metadata":{"_buckets":{"deposit":"84143109-00ed-4194-a948-f14c9804016f"},"_deposit":{"created_by":3,"id":"3563","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3563"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003563","sets":["14:2667:2675:2679:2683","21:2669:2677:2681:2684"]},"author_link":["4269","4272","4268","4271","4270"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Involvement of the oxidized low-density lipoprotein/lectin-like oxidized low-density lipoprotein receptor-1system to joint cartilage degeneration"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2012. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究の目的は、酸化低密度リボ蛋白(酸化LDL)が軟骨組織の老化変性に関与することを、レクチン様酸化LDL受容体-1(LOX-1) knockout (KO) mouseを用いてin vivoで明らかにすることである.  トレッドミルを用いてCB57/BL6マウスを強制走行(週3回、2週間に1Km)させると、非侵襲的に変形性膝関節症を生じることが明らかになった.  また、これらの変化は走行週数、一回当たり走行距離により調節可能であった.  そこでLOX-1 KO mouseを強制走行させ、膝軟骨変性における酸化LDL/LOX-1系の関与を明らかにすることを試みたが、両マウスの変形の程度において2週間の走行では有意な差は無かった.  次に、6ヶ月、1年、1.5年と長期間自然飼育したwild typeとKO mouseの膝軟骨変性を観察した.  その結果、飼育開始後1、1.5年において軟骨変性がKO mouseにおいて有意に抑制されていた.  さらに、Zymosan関節内投与による関節炎モデルマウスにおいても、LOX-1 KOにより滑膜炎症、軟骨変性の抑制が認められ、LOX-1と関節症変化の関連が示された.  以上の結果より、酸化LDL、および酸化LDLの受容体LOX-1は血管内皮細胞の老化促進因子であるのみならず軟骨細胞の老化変性にも関与を示し、変形性関節症の薬物的治療に新たな可能性を示すことができたと考える.    研究成果の概要（英文）：Purpose of this study is to demonstrate involvement of the ox-LDL/LOX-1 system to joint cartilage degeneration in vivo using LOX-1 knockout (KO) mouse. To develop a nonsurgical murine knee osteoarthritis model, mechanical load is applied by forced running on a treadmill, which was controlled by changing number of forced running periods and running time. Changes in the degeneration score increased significantly in a time- and dose-dependent manner. However, LOX-1 KO did not show significant protective effects in this OA model after 2-week forced running, compared with wild type mice. While, long-term observation (1 and 1.5 year) of the KO mice and wild type mice bred conventionally in cages revealed significant difference in cartilage degeneration. Furthermore, LOX-1 KO suppressed synovitis and cartilage degeneration in zymosan induced arthritis. These results indicate that the ox-LDL/L0X-1 system is involved to joint cartilage degeneration in vivo. In conclusion, the ox-LDL/LOX-1 system may play some role not only in endothelial dysfunction of atherosclerosis but also in cartilage degeneration.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"機関番号:34419;   研究種目：基盤研究（C）;  研究期間：2010～2012;   課題番号：22591694;   研究分野：医歯薬学;   科研費の分科・細目：外科系臨床医学・整形外科学","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/00273441.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/00330283.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/10635569.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/30243033.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/40460901.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"連携研究者"},{"subitem_text_value":"連携研究者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"AKAGHI, MASAO"},{"subitem_text_language":"en","subitem_text_value":"ASADA, SHIGEKI"},{"subitem_text_language":"en","subitem_text_value":"HASHIMOTO, KAZUHIKO"},{"subitem_text_language":"en","subitem_text_value":"SAWAMURA, TATSUYA"},{"subitem_text_language":"en","subitem_text_value":"TERAMURA, TAKESHI"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 教授"},{"subitem_text_value":"近畿大学医学部; 講師"},{"subitem_text_value":"近畿大学医学部附属病院; 助教"},{"subitem_text_value":"国立循環器病センター(研究所)脈管整理部; 部長"},{"subitem_text_value":"近畿大学医学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"Kinki University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"赤木,  將男"},{"creatorName":"アカギ, マサオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"朝田,  滋貴"},{"creatorName":"アサダ, シゲキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"橋本,  和彦"},{"creatorName":"ハシモト, カズヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"沢村,  達也"},{"creatorName":"サワムラ, タツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"寺村,  岳士"},{"creatorName":"テラムラ, タケシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_22591694seika.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_22591694seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3563/files/KAKEN_22591694seika.pdf"},"version_id":"382d0779-f7e3-4e3c-8431-6c6ae50131a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"医療・福祉","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞・組織","subitem_subject_scheme":"Other"},{"subitem_subject":"脂質","subitem_subject_scheme":"Other"},{"subitem_subject":"トランスレーショナルリサーチ","subitem_subject_scheme":"Other"},{"subitem_subject":"老化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"酸化LDL/LOX-1系の軟骨変性への関与","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酸化LDL/LOX-1系の軟骨変性への関与"}]},"item_type_id":"8","owner":"3","path":["2683","2684"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-07-25"},"publish_date":"2013-07-25","publish_status":"0","recid":"3563","relation_version_is_last":true,"title":["酸化LDL/LOX-1系の軟骨変性への関与"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:31:25.912917+00:00"}