{"created":"2023-06-20T16:34:20.105021+00:00","id":3506,"links":{},"metadata":{"_buckets":{"deposit":"e13f8e18-bb51-42c2-a649-f7bc4fe88acd"},"_deposit":{"created_by":3,"id":"3506","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3506"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003506","sets":["14:2667:2675:2679:2680","21:2669:2677:2681:2682"]},"author_link":["3380"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Analysis of regulatory mechanisms of C/N—balance by plastid signaling using sugar—sensitive mutants"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2012. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要(和文): プラスチド型インベルターゼ(INV-E)変異株(sicy-192)は、ショ糖を含む培地上で緑化が抑制され、光合成系および窒素同化系遺伝子群の発現が野生株とは異なる。そこで、プラスチドから核への逆行性シグナリング(プラスチドシグナリング)のマスターレギュレーターであるGENOME UNCOUPLED1欠損株(gun1-101)とsicy-192の二重変異株を作出し解析した結果、sicy-192で見られた光合成系遺伝子群の発現抑制および窒素同化系遺伝子群の発現誘導は、二重変異株において部分的に回復していたことから、sicy-192変異はプラスチド遺伝子発現を抑制し、GUN1を介したプラスチドシグナリングを活性化することにより種々の核コード遺伝子の発現を変化させることが明らかになった。        研究成果の概要(英文): We recently identified an Arabidopsis gain-of-function mutant of plastidic invertase (INV-E), sicy-192, as a sucrose sensitive mutant, and found that INV-E participates in regulating the carbon/nitrogen (C/N) balance. Interestingly, PGE was suppressed in the mutants on treatment with sucrose, suggesting that INV-E regulates C/N balance via the plastid signaling. We created and analyzed double mutants of sicy-192 and genome uncoupled 1 (gunl-101), which lacks GUN1, a master regulator of the plastid signaling. On treatment with sucrose, the expression of photosynthesis- and nitrogen assimilation-related genes was lower and higher, respectively, in the sicy-192 mutants compared to the wild-type plants. Interestingly, the perturbation of C/N balance was recovered by the disruption of GUN1, suggesting an involvement of INV-E in the GUN1-mediated plastid signaling.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"機関番号: 34419;   研究種目：若手研究(B);   研究期間：2011～2012;   課題番号：23780108;   研究分野：農学;    科研費の分科・細目：農芸化学・応用生物化学","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/70340768.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"TAMOI, MASAHIRO"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部; 准教授"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田茂井,  政宏"},{"creatorName":"タモイ, マサヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_23780108seika.pdf","filesize":[{"value":"690.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_23780108seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3506/files/KAKEN_23780108seika.pdf"},"version_id":"57993227-60b3-4023-825a-408182c94057"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"植物生化学","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"糖応答変異体を用いたC/Nバランス制御および葉緑体-核間シグナル伝達機構の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"糖応答変異体を用いたC/Nバランス制御および葉緑体-核間シグナル伝達機構の解明"}]},"item_type_id":"8","owner":"3","path":["2680","2682"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-07-19"},"publish_date":"2013-07-19","publish_status":"0","recid":"3506","relation_version_is_last":true,"title":["糖応答変異体を用いたC/Nバランス制御および葉緑体-核間シグナル伝達機構の解明"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:33:16.081064+00:00"}