{"created":"2023-06-20T16:34:17.019163+00:00","id":3435,"links":{},"metadata":{"_buckets":{"deposit":"656e6393-5ab9-43b5-89ce-9b30eb181519"},"_deposit":{"created_by":3,"id":"3435","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3435"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003435","sets":["14:2667:2668:2987","21:2669:2670:2988"]},"author_link":["4060"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Increased ectodomain shedding of lung-epithelial cell adhesion molecule 1 as a cause of increased alveolar cell apoptosis in emphysema"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2014. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文): 肺気腫の原因である蛋白分解酵素活性上昇と肺胞上皮アポトーシスを結び付ける分子機序は不明であった. 肺胞上皮の接着分子CADM1の機能的制御に細胞外切断（shedding）がある. ウエスタン法にて肺気腫ではsheddinが亢進し, TUNEL法にて肺胞上皮アポトーシスの上昇が判明した. ヒト肺上皮細胞株NCI-H441細胞においてCADM1のsheddingを惹起させた所, shedding産物であるαCTFがミトコンドリアに局在し, アポトーシスが上昇することを見出した. 肺気腫ではCADM1のsheddingが亢進し, αCTFがミトコンドリアに集積して肺胞上皮アポトーシスを促進していると考えられた.                     研究成果の概要（英文): Alveolar epithelial cell apoptosis and proteolysis play central roles in the pathogenesis of emphysema, but molecular mechanisms underlying these two events are not yet clearly understood. CADM1 is a lung-epithelial cell adhesion molecule and generates membrane-associated C-terminal fragments (CTFs) through protease-mediated ectodomain shedding. Western blot analyses revealed that CADM1-CTFs increased in human emphysematous lungs in association with increased ectodomain shedding. Increased apoptosis of alveolar epithelial cells in emphysematous lungs was confirmed by TUNEL assays. NCI-H441 lung epithelial cells expressing mature CADM1 but not CTFs were induced to express αCTF by shedding inducers; phorbol ester and trypsin. Immunofluorescence and TUNEL assays revealed that CADM1-αCTF was localised to mitochondria and increased cell apoptosis. CADM1 shedding appeared to cause alveolar cell apoptosis in emphysematous lungs by producing αCTF that accumulated in mitochondria.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(B);  研究期間：2013～2014;   課題番号：25860302;   研究分野：実験病理;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/60632718.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"HAGIYAMA, Mitsuru"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 助教"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"萩山,  満"},{"creatorName":"ハギヤマ, ミツル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4060","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60632718","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_25860302seika.pdf","filesize":[{"value":"516.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_25860302seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3435/files/KAKEN_25860302seika.pdf"},"version_id":"9c7e46ad-be6a-459d-9d1a-1fb1829ff8aa"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"細胞障害","subitem_subject_scheme":"Other"},{"subitem_subject":"接着分子","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテアーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"アポトーシス","subitem_subject_scheme":"Other"},{"subitem_subject":"肺気腫","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"肺気腫の新規発症機序 : 接着分子CADM1の細胞内断片による肺胞上皮アポトーシス","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"肺気腫の新規発症機序 : 接着分子CADM1の細胞内断片による肺胞上皮アポトーシス"}]},"item_type_id":"8","owner":"3","path":["2987","2988"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-10-23"},"publish_date":"2015-10-23","publish_status":"0","recid":"3435","relation_version_is_last":true,"title":["肺気腫の新規発症機序 : 接着分子CADM1の細胞内断片による肺胞上皮アポトーシス"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:35:42.315662+00:00"}