{"created":"2023-06-20T16:34:16.749453+00:00","id":3429,"links":{},"metadata":{"_buckets":{"deposit":"ee34a55f-7c7e-4b61-8228-b60738ff9f55"},"_deposit":{"created_by":3,"id":"3429","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3429"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003429","sets":["14:2667:2668:2987","21:2669:2670:2988"]},"author_link":["4045","4044","4043"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Novel development and applications of the reagents for chemical scission of targetted proteins as chemical scissors"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2014. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文): 本研究の目的は, 標的蛋白化学的切除試薬の開発と臨床応用であった. 理由は, 化学的に温和な反応条件で迅速に蛋白破壊可能な試薬が, 疾患治療および病因究明に必要なためである. 方法と原理 : 開発試薬の蛋白過酸化イソインドールは, 蛋白をo-phthalaldehydeとチオール化合物でイソインドールへ変換し, 続いて過酸化水素で過酸化して作成した. 結果 : 蛋白過酸化イソインドール形成は, 蛍光画像撮影で証明した. 蛋白過酸化イソインドールの酸化で発光が生じた. 発光後, その蛍光画像は消失した（蛋白破壊の証明）. 血清蛋白分画のバンド蛋白も, 切除できた. 結論: 本試薬により, 蛋白破壊が同時発光モニターしながら可能となった.            研究成果の概要（英文): Development of the novel reagent for chemical scission of targeted proteins and its clinical application　were studied. The reagents those demolish proteins under mild chemical conditions have been requested for treatment of patients and research of diseases. The reagents were protein peroxyisoindoles of proteins, and were prepared by the reaction with hydrogen peroxide following to the reaction with o-phthalaldehyde and thiol compounds. The production of protein peroxyisoindole were confirmed by fluorescence imaging. The peroxyisoindole was oxidized to emit light, accompanying of disappearance of the fluorescence image. This meant demolition of proteins. The reagent of peroxyisoindole was also applied to serum protein fractionated by electrophoresis resulting in successful cleavage. In conclusion, the novel reagents of proteins allowed simultaneous protein scission and monitoring of the accompanying chemiluminescence.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：挑戦的萌芽研究;  研究期間：2013～2014;   課題番号：25650038;   研究分野：臨床検査医学, 内科学, 分析化学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/50164687.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/40416318.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究協力者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Nozaki, Osamu"},{"subitem_text_language":"en","subitem_text_value":"SHIZUMA, Motohiro"},{"subitem_text_language":"en","subitem_text_value":"KAWAMOTO, Hiroko"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部附属病院; 講師"},{"subitem_text_value":"地方独立行政法人 大阪市立工業研究所生物生活材料研究部; 研究員"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Osaka Municipal Technical Research Institute"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野崎,  修"},{"creatorName":"ノザキ, オサム","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4043","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40416318","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"静間,  基博"},{"creatorName":"シズマ, モトヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4044","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河本,  裕子"},{"creatorName":"カワモト, ヒロコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4045","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_25650038seika.pdf","filesize":[{"value":"878.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_25650038seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3429/files/KAKEN_25650038seika.pdf"},"version_id":"d02d0d05-dd15-4dbf-a492-b308fafea1a4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"蛋白切除","subitem_subject_scheme":"Other"},{"subitem_subject":"蛋白過酸化イソインドール","subitem_subject_scheme":"Other"},{"subitem_subject":"化学発光","subitem_subject_scheme":"Other"},{"subitem_subject":"蛍光画像測定","subitem_subject_scheme":"Other"},{"subitem_subject":"質量解析","subitem_subject_scheme":"Other"},{"subitem_subject":"試薬開発","subitem_subject_scheme":"Other"},{"subitem_subject":"血清蛋白分画","subitem_subject_scheme":"Other"},{"subitem_subject":"o-phthalaldehyde","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"標的蛋白化学的切除試薬（ケミカルシザーズ）の開発と応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"標的蛋白化学的切除試薬（ケミカルシザーズ）の開発と応用"}]},"item_type_id":"8","owner":"3","path":["2987","2988"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-10-23"},"publish_date":"2015-10-23","publish_status":"0","recid":"3429","relation_version_is_last":true,"title":["標的蛋白化学的切除試薬（ケミカルシザーズ）の開発と応用"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:35:53.975048+00:00"}