{"created":"2023-06-20T16:34:07.515888+00:00","id":3213,"links":{},"metadata":{"_buckets":{"deposit":"c5380d6e-3232-48da-abfe-7df2a813ee06"},"_deposit":{"created_by":3,"id":"3213","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3213"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003213","sets":["14:2667:2685:2686","21:2669:2687:2688"]},"author_link":["3658","3659"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Development of novel screening system for enzyme-displaying yeasts using calcium-alginate based beads."}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費補助金研究成果報告書 (2011. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：我々は、これまでの研究で、アルギン酸カルシウムビーズ中に核酸、タンパク質、あるいは細胞を封入する技術開発を行ってきた。本技術を用いれば、ビーズ内に酵母細胞を包摂し、この内で個々に酵素反応を起こさせることにより、反応後の標識分子の溶液中への拡散を防ぎつつ、活性の高い表層提示酵母細胞をビーズごとセルソーティングにより取得できると考えられる。そこで本研究では, 近年発展の著しい, コンビナトリアルバイオエンジニアリングの手法を用いた、変異体タンパク質ライブラリーの大量作製とその大規模なスクリーニングに資する新しい表層提示酵母細胞のスクリーニング方法の開発を行った。              研究成果の概要（英文）：In this study, a novel screening method for enzyme-displaying yeasts were investigated. Enzyme-displayed yeast cells were captured in the micro-sized calcium alginate beads each by each using newly developed reverse micelle method to prevent diffusion of hydrolyzed fluorescent substrates. Adopting flow sorting to these captured cells, active cells were successfully enriched. This system would be a useful method for high speed screening of yeast cells which display various hydrolyzing enzymes with high activity.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究（C）;  研究期間：2009～2011;   課題番号：21560812;   研究分野：工学;   科研費の分科・細目：プロセス工学、生物機能・バイオプロセス","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/20243496.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/90436551.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"KAJIYAMA, SHINICHIRO"},{"subitem_text_language":"en","subitem_text_value":"TANAKA, TSUTOMU"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 教授"},{"subitem_text_value":"神戸大学自然科学系先端融合研究環重点研究部; 准教授"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"梶山,  慎一郎"},{"creatorName":"カジヤマ, シンイチロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"田中,  勉"},{"creatorName":"タナカ, ツトム","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_21560812seika.pdf","filesize":[{"value":"299.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_21560812seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3213/files/KAKEN_21560812seika.pdf"},"version_id":"c84aaa92-4ef0-4399-ba5f-cf40a9fe21ba"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"シングルセル","subitem_subject_scheme":"Other"},{"subitem_subject":"酵母表層工学","subitem_subject_scheme":"Other"},{"subitem_subject":"活性スクリーニング","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞包摂","subitem_subject_scheme":"Other"},{"subitem_subject":"ソーティング","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"シングルセル包摂技術を用いた酵母表層提示酵素の高速活性測定系の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シングルセル包摂技術を用いた酵母表層提示酵素の高速活性測定系の開発"}]},"item_type_id":"8","owner":"3","path":["2686","2688"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-22"},"publish_date":"2012-10-22","publish_status":"0","recid":"3213","relation_version_is_last":true,"title":["シングルセル包摂技術を用いた酵母表層提示酵素の高速活性測定系の開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:42:57.904398+00:00"}