{"created":"2023-06-20T16:34:07.218474+00:00","id":3206,"links":{},"metadata":{"_buckets":{"deposit":"7878b5f9-ad50-407d-9298-767c9508a1e6"},"_deposit":{"created_by":3,"id":"3206","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3206"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003206","sets":["14:2667:2685:2686","21:2669:2687:2688"]},"author_link":["3647","3649","3648"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Development of cell microarray as devices for cell diagnosis, regulation of cell function, and recovery of target cells"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費補助金研究成果報告書 (2011. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：サイズやパターンを制御したガラススポットに，温度で細胞接着・剥離を制御する素材と周囲を細胞非接着素材としたマイクロアレイを調製した．接着細胞の90％以上が温度刺激で回収できた．また，スポット上に抗体結合部位を修飾し，細胞特異的な抗体で目的細胞を選択固定化し，開発装置を用いて高速診断を可能とした．0.001％のヒト人工染色体(HAC)含微小核細胞の受容細胞への融合を，最大0.1％まで効率化した．細胞マイクロアレイと装置で，診断／制御と分取用デバイスとして実用化可能であることを示した．　　　　　　  　研究成果の概要（英文）： Four types of microarrays(MA: well sizes＝20,30,40,50μm; pitch＝100μm) spotted with poly(N-isopropylacrylamide;PNiPAAm) or biocompatible MPC polymers having anti-body as a specific binding for target cells were prepared by surface-initiated polymerization of NiPAAm or MPC / N-succinimidylacrylate on the glass surface of MA. Poly(MPC) was coated around spots on the MAs . Selective cell attachment was achieved by using HL60 cells or induced human pluripotent stem(iPS) cells as a typical cell on the MAs after being reacted with anti-body as a specific binding site for targeting cells. High-throughput diagnosis of attached cells on the MA was also succeeded by using developed equipments. Over 90％ attached HeLa cells on the PNiPAAm coated spots of the MA were efficiently exfoliated by decreasing cell cultured temp. from 37℃to 25℃. Microcell(MC)s containing Human Artifical Chromosome(HAC) and U937 as white blood cell were higher fused on the MCs immobilized MA with a maximum of 0.1％ than that of suspension cell fusion(0.001％). The MAs were of useful and for practical uses as devices for cell diagnosis, regulation of cell function, and recovery of target cells.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2009～2011;   課題番号：21500457;   研究分野：総合領域;   科研費の分科・細目：人間医工学・医用システム","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/10196602.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/00166737.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/d/r/90183102.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"連携研究者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"SHIRAISHI, KOHEI"},{"subitem_text_language":"en","subitem_text_value":"YAMADA, YASUE"},{"subitem_text_language":"en","subitem_text_value":"HOSOYA, HIROSHI"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学工学部; 教授"},{"subitem_text_value":"近畿大学工学部; 教授"},{"subitem_text_value":"広島大学大学院理学研究科; 教授"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"白石,  浩平"},{"creatorName":"シライシ, コウヘイ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3647","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90183102","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"山田,  康枝"},{"creatorName":"ヤマダ, ヤスエ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3648","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"細谷,  浩史"},{"creatorName":"ホソヤ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3649","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_21500457seika.pdf","filesize":[{"value":"771.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_21500457seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3206/files/KAKEN_21500457seika.pdf"},"version_id":"7c8f0785-3beb-4725-ad34-8884349247fb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"検査・診断システム","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞・組織工学","subitem_subject_scheme":"Other"},{"subitem_subject":"生体適合性材料","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"診断／制御と分取デバイスとしての細胞マイクロアレイの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"診断／制御と分取デバイスとしての細胞マイクロアレイの開発"}]},"item_type_id":"8","owner":"3","path":["2686","2688"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-01"},"publish_date":"2012-10-01","publish_status":"0","recid":"3206","relation_version_is_last":true,"title":["診断／制御と分取デバイスとしての細胞マイクロアレイの開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:43:10.222278+00:00"}