{"created":"2023-06-20T16:34:01.563055+00:00","id":3104,"links":{},"metadata":{"_buckets":{"deposit":"c7be1e0f-1fe2-4895-add3-46a0f4ff0e32"},"_deposit":{"created_by":3,"id":"3104","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3104"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003104","sets":["14:2667:2689:2690","21:2669:2691:2692"]},"author_link":["3389"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Development of genetically engineered animals by use of non-B DNA structure for highly and stably expression of transgene"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費補助金研究成果報告書 (2010. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：高効率・安定的な遺伝子改変マウスの開発をめざし、非B型DNA人工配列によるRNAポリメラーゼII型ならびにIII依存性の転写活性の賦活化作用について検討した。その結果、非B型DNA人工配列モデルとしたT20配列について、(1)ES細胞におけるPolII型転写活性の賦活化作用、(2)ES細胞の分化過程における作用の安定性とクロマチン構造の動態、(3)T20配列導入マウスの作製に関する新たな知見が得られた。   研究成果の概要（英文）：This study examined the function of non-B DNA sequence on the activation of transcription by RNAPII and/or RNAPIII to develop transgenic animals with highly efficient and stable expression of transgene. The results may be summarized as follows: T20 (180bp artificial curved DNA) as a model of non-B DNA sequence showed (1) the activation of the transcription by RNAPII in mouse ES cells, (2) the stability of transcription and chromatin dynamics during in vitro differentiation of ES cells, and (3) the possibility of the production of T20-driven transgenic mice.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：新学術領域研究(課題提案型);   研究期間：2008～2010;   課題番号：20200032;    研究分野：農学、複合領域;    科研費の分科・細目：畜産学・獣医学・発生工学、ゲノム科学・ゲノム工学","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/r/10322265"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"MITANI, TASUKU"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先端技術総合研究所; 准教授"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三谷,  匡"},{"creatorName":"ミタニ, タスク","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_20200032seika.pdf","filesize":[{"value":"238.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_20200032seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3104/files/KAKEN_20200032seika.pdf"},"version_id":"ae04a060-94b6-423d-bea5-907c2f41e2c9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"非Ｂ型ＤＮＡ配列を利用した遺伝子発現制御による高効率・安定的遺伝子改変動物の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"非Ｂ型ＤＮＡ配列を利用した遺伝子発現制御による高効率・安定的遺伝子改変動物の開発"}]},"item_type_id":"8","owner":"3","path":["2690","2692"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-06-28"},"publish_date":"2011-06-28","publish_status":"0","recid":"3104","relation_version_is_last":true,"title":["非Ｂ型ＤＮＡ配列を利用した遺伝子発現制御による高効率・安定的遺伝子改変動物の開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:46:32.787497+00:00"}