{"created":"2023-06-20T16:34:00.118656+00:00","id":3073,"links":{},"metadata":{"_buckets":{"deposit":"e6655e2b-ae84-4d64-a61c-6e3969d7b933"},"_deposit":{"created_by":3,"id":"3073","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3073"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00003073","sets":["14:2667:2695:2699","21:2669:2697:2700"]},"author_link":["3360","3357","3359","3358","3356","3361"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Mechanical stress loaded on articular cartilage explant"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費補助金研究成果報告書 (2009. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"関節軟骨移植（ACI）は運動器疾患に対する代表的な再生医療である．本研究では，ACI の力学的環境に関する検討を行なった．培養軟骨片への圧迫負荷により，プロテオグリカン（PG）合成の抑制と活性酸素量の増加を認めた．そこで，酸化ストレスの緩和を目的にヒアルロン酸(HA)の添加を検討した．HA は活性酸素を抑制し，PG 産生量を回復させた．このことから，ACI の環境改善におけるHA 投与の有用性が示唆された．（英文） Introduction : To repair the articular cartilage defect, cartilage explant is useful method. The key leading to the success of this regenerative medicine is to regulate the mechanical stress loaded on articular cartilage explant. Intraarticular hyaluronan (HA) injection is an effective treatment for cartilage degeneration. The purpose of this study is to determine the effect of mechanical stress loaded on the cartilage explant and possible usefulness of HA in this system. Method s : Mechanical compression was loaded on the bovine cartilage using the Biopress system. Proteoglycan (PG) and reactive oxygen species (ROS) synthesis were measured with [35S] incorporation and fluorescent dye, respectively. Accumulation of peroxynitrite was determined with western blotting using nitrotyrosine antibody.Result s : Mechanical compression inhibited PG synthesis and enhanced ROS. Externally added HA reversed stress-inhibited PG synthesis and attenuated ROS synthesis. HA also significantly decreased the expression of nitrotyrosine.C o n c l u s i o n s : Mechanical stress is essential factor to degradate the explanted cartilage and ROS play an important role. HA neutralized stress-enhanced ROS synthesis and resulted in the reverse of PG synthesis. These data suggest that HA plays anabolic effect as an antioxidant.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究（Ｃ）研究期間：2007～2009課題番号：19591770研究分野：医歯薬学科研費の分科・細目：外科系臨床医学・整形外科学","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://rns.nii.ac.jp/nr/1000050201744"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://rns.nii.ac.jp/nr/1000020258031"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://rns.nii.ac.jp/nr/1000020258031"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://rns.nii.ac.jp/nr/1000000330283"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://rns.nii.ac.jp/nr/1000080330314"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://rns.nii.ac.jp/nr/1000040460901"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"FUKUDA, KANJI"},{"subitem_text_language":"en","subitem_text_value":"OTANI, KAZUHIRO"},{"subitem_text_language":"en","subitem_text_value":"NONAKA, TOGO"},{"subitem_text_language":"en","subitem_text_value":"ASADA, SHIGEKI"},{"subitem_text_language":"en","subitem_text_value":"NISHISAKA, FUMIAKI"},{"subitem_text_language":"en","subitem_text_value":"TERAMURA, TAKESHI"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学・医学部・教授"},{"subitem_text_value":"近畿大学・医学部・講師"},{"subitem_text_value":"近畿大学・医学部・講師"},{"subitem_text_value":"近畿大学・医学部・講師"},{"subitem_text_value":"近畿大学・医学部・講師"},{"subitem_text_value":"近畿大学・医学部附属病院・助教"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福田,  寛二"},{"creatorName":"フクダ, カンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"大谷,  和裕"},{"creatorName":"オオタニ, カズヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"野中,  藤吾"},{"creatorName":"ノナカ, トウゴ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"朝田,  滋貴"},{"creatorName":"アサダ, シゲキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西坂,  文章"},{"creatorName":"ニシサカ, フミアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"寺村,  岳士"},{"creatorName":"テラムラ, タケシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"detail","filename":"KAKEN_19591770seika.pdf","filesize":[{"value":"382.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_19591770seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/3073/files/KAKEN_19591770seika.pdf"},"version_id":"91b284b3-79e4-4718-ba6e-a7512880f821"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"軟骨","subitem_subject_scheme":"Other"},{"subitem_subject":"ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"活性酸素","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"関節軟骨移植の力学的環境に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"関節軟骨移植の力学的環境に関する研究"}]},"item_type_id":"8","owner":"3","path":["2699","2700"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-06"},"publish_date":"2010-07-06","publish_status":"0","recid":"3073","relation_version_is_last":true,"title":["関節軟骨移植の力学的環境に関する研究"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T01:47:32.898391+00:00"}