{"created":"2023-06-20T16:50:49.331307+00:00","id":23379,"links":{},"metadata":{"_buckets":{"deposit":"2c70d4db-6dbe-4665-8b20-567a0871ed14"},"_deposit":{"created_by":3,"id":"23379","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"23379"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00023379","sets":["14:2667:4819"]},"author_link":["43471"],"control_number":"23379","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"15","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2021)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K05722/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究は, キャリアータンパク質担持型共有結合性異性化酵素阻害剤 (クロスリンク剤) を設計および創製し, 異性化酵素の触媒機構およびキャリアータンパク質認識機構を明らかにすることを目的とした. 本研究では, クロスリンク剤の創製に成功した. また, チロシジン合成酵素TycAをモデルとして、異性化酵素の活性部位変異体を作製した. さらに, 本クロスリンク剤を利用することでGlu882がクロスリンク剤との反応部位であることが明らかとなった. このことから, Glu882が異性化反応においてアミノ酸のα位水素を引き抜く塩基触媒として作用していることが示唆された.\n研究成果の概要（英文）： Nonribosomal peptide synthetases (NRPSs) unique molecular factories can produce peptides with nonproteinogenic D-amino acids in which the epimerization (E) domain catalyzes the conversion of L-amino acids to D-amino acids, but its mechanism remains not fully understood. Here, we describe the development of pantetheine crosslinking probes that mimic the natural substrate L-Phe of the initiation module of tyrocidine synthetase, TycA, to elucidate and study the catalytic residues of the E domain. Mechanism-based crosslinking assays and MALDI-TOF MS were used to identify both H743 and E882 as the crosslinking site residues, demonstrating their roles as catalytic bases. Mutagenesis studies further validated these results and allowed the comparison of reactivity between the catalytic residues, concluding that glutamate acts as the dominant nucleophile in the crosslinking reaction, resembling the deprotonation of the Ca-H of amino acids in the epimerization reaction.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C); 研究期間：2019～2021; 課題番号：19K05722; 研究分野：ケミカルバイオロジー; 科研費の分化・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Ishikawa, Fumihiro"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学薬学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石川, 文洋"},{"creatorName":"イシカワ, フミヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-12-05"}],"displaytype":"detail","filename":"19K05722seika.pdf","filesize":[{"value":"718.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"19K05722seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/23379/files/19K05722seika.pdf"},"version_id":"f48c7a25-a07b-4dd6-bb2b-0d8cffacae85"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"非リボソームペプチド合成酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"異性化酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"キャリアータンパク質","subitem_subject_scheme":"Other"},{"subitem_subject":"共有結合性異性化酵素阻害剤","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質間相互作用","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"NRPS異性化酵素機能を解明する共有結合型分子ツールの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"NRPS異性化酵素機能を解明する共有結合型分子ツールの開発","subitem_title_language":"ja"},{"subitem_title":"Developing crosslinkers specific for epimerization domain in initiation modules to evaluate mechanism","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4819"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-12-05"},"publish_date":"2022-12-05","publish_status":"0","recid":"23379","relation_version_is_last":true,"title":["NRPS異性化酵素機能を解明する共有結合型分子ツールの開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-12T05:58:36.304386+00:00"}