{"created":"2023-06-20T16:50:45.455343+00:00","id":23319,"links":{},"metadata":{"_buckets":{"deposit":"801407f8-2fda-49be-b3f0-b5c38649eee2"},"_deposit":{"created_by":3,"id":"23319","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"23319"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00023319","sets":["14:2667:4819"]},"author_link":["30522","30524","30523","43525"],"control_number":"23319","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2021)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K10020/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要(和文):耳介形状の軟骨再生において、生体内で3次元耳介形状を維持する方法を確立することを目的とした。実験では、PGA ナノファイバー(nanoPGA)とポリ-ε-カプロラクトン(PCL)を組み合わせた複合型吸収性スキャフォールドをエタノール処理して播種細胞の親水性を高め、軟骨再生における効果を検討した。その結果、スキャフォールドの生体内移植後20週間目において、有意に高いSOX5遺伝子発現が誘導され、サフラニンO陽性染色部位はスカフォールド内部に拡大していることが判明した。エタノールによる複合型吸収性スカフォールドの表面改質は、経時的に軟骨の基質産生を促進し耳介形状軟骨再生における有効性が示唆された。\n研究成果の概要(英文): Human chondrocytes were seeded onto three-dimensional bioresorbable composite scaffolds (PCL with surface coverage of nanoPGA) either with or without ethanol pretreatment and then implanted into athymic mice for 10 and 20 weeks. After implantation for 10 weeks, constructs of human auricular chondrocytes seeded onto ethanol-treated scaffolds were covered with glossy cartilage. RT-qPCR analyses of chondrocytes grown on ethanol-treated scaffolds showed greater expression levels for several cartilage-related genes compared to cells developed on untreated scaffolds with statistically significantly increased SRY-box transcription factor 5 (SOX5) and decreased interleukin-1α (inflammation-related) expression levels. Findings suggest enhancing nanoPGA/PCL scaffold hydrophilicity utilizing ethanol improves the cellular microenvironment and cartilage regeneration in tissue-engineered auricle constructs.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目:基盤研究(C); 研究期間:2019~2021; 課題番号:19K10020; 研究分野:形成外科学; 科研費の分化・細目:","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Isogai, Noritaka"},{"subitem_text_language":"en","subitem_text_value":"Morotomi, Tadaaki"},{"subitem_text_language":"en","subitem_text_value":"Kusuhara, Hirohisa"},{"subitem_text_language":"en","subitem_text_value":"Wada, Yoshitaka"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 教授"},{"subitem_text_value":"近畿大学医学部; 准教授"},{"subitem_text_value":"近畿大学医学部; 講師"},{"subitem_text_value":"和歌山県立医科大学医学部; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"},{"subitem_text_value":""}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"磯貝, 典孝"},{"creatorName":"イソガイ, ノリタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"諸富, 公昭"},{"creatorName":"モロトミ, タダアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"楠原, 廣久"},{"creatorName":"クスハラ, ヒロヒサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"和田, 仁孝"},{"creatorName":"ワダ, ヨシタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-12-05"}],"displaytype":"detail","filename":"19K10020seika.pdf","filesize":[{"value":"92.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"19K10020seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/23319/files/19K10020seika.pdf"},"version_id":"5c13b4d7-340c-4089-b4c5-53746e18c189"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ethanol treatment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"scaffold","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cartilage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tissue engineering","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ear","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrophilicity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"表面改質した複合型吸収性ナノファイバー足場を用いた耳介形状軟骨の3次元再生誘導","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"表面改質した複合型吸収性ナノファイバー足場を用いた耳介形状軟骨の3次元再生誘導","subitem_title_language":"ja"},{"subitem_title":"Ethanol pretreatment of nanoPGA/PCL composite scaffolds enhances human chondrocyte development in the tissue-engineered auricle constructs","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4819"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-12-05"},"publish_date":"2022-12-05","publish_status":"0","recid":"23319","relation_version_is_last":true,"title":["表面改質した複合型吸収性ナノファイバー足場を用いた耳介形状軟骨の3次元再生誘導"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-02T06:22:04.463966+00:00"}