@techreport{oai:kindai.repo.nii.ac.jp:00023306,
 author = {坂井, 和子 and 西尾, 和人 and 海堀, 昌樹},
 month = {},
 note = {https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K07722/, 研究成果の概要（和文）：ゲノムワイド一塩基多型アレイから得られたコピー数変動及びB-アレル頻度に基づいたClonal composition数（CC数）を腫瘍内不均一性の指標とし、肝細胞癌（HCC）組織におけるCC数の多寡による腫瘍内不均一性を有する腫瘍の分子病態を解明することを目的した。切除可能な肝細胞癌の腫瘍組織検体36例を解析した結果、腫瘍内不均一性の高いpoly-CC腫瘍と均質なmono-CC腫瘍に分類された。Poly-CC腫瘍を有する患者はMono-CC腫瘍の患者に比べて有意に無再発生存期間が不良であり、細胞周期関連経路の遺伝子に富み、より活発な細胞増殖を示すことが示された。
研究成果の概要（英文）： Tumor heterogeneity based on copy number variations is associated with the evolution of cancer and its clinical grade. Clonal composition (CC) represents the number of clones based on the distribution of B-allele frequency (BAF) obtained from a genome-wide single nucleotide polymorphism (SNP) array. A higher CC number represents a high degree of heterogeneity. Somatic mutations, total transcriptome, and copy number variation were analyzed in patients with resectable HCC. The samples were classified the heterogeneous tumors as poly-CC (n=26) and the homogeneous tumors as mono-CC (n=8). The patients with poly-CC had a higher rate of early recurrence and a significantly shorter recurrence-free survival period than the mono-CC patients. A transcriptome analysis showed that cell cycle-related pathways were enriched in the poly-CC tumors compared with the mono-CC tumors. Poly-CC HCC is highly proliferative and has a high risk of early recurrence., 研究種目：基盤研究(C); 研究期間：2019～2021; 課題番号：19K07722; 研究分野：医学; 科研費の分化・細目：, application/pdf},
 title = {肝細胞癌における腫瘍内不均一性の定量的評価による病態の解明},
 year = {2021},
 yomi = {サカイ, カズコ and ニシオ, カズト and カイボリ, マサキ}
}