{"created":"2023-06-20T16:50:40.856081+00:00","id":23244,"links":{},"metadata":{"_buckets":{"deposit":"fc8320d0-d710-4ada-8c58-ece046ba36b3"},"_deposit":{"created_by":3,"id":"23244","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"23244"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00023244","sets":["14:2667:4819"]},"author_link":["43543","43544","43545","43546","43547","43548","43549"],"control_number":"23244","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2021)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K12751/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：ヒト応答予測の向上を目指し、酸素非依存性の電気化学バイオセンサでのモニタリングを利用することで、細胞応答を非侵襲で評価するためのシステム開発を検討した。肝細胞については、電気化学グルコースセンサに好気的培養手法を組み合わせることで、生体内の門脈近傍の肝組織と同質の糖代謝･糖新生の現象を電流応答で記述できた。この他にも、アレルギー研究で広く利用される好塩基球様細胞株RBL-2H3が免疫刺激によって遊離するヒスタミンの電気化学モニタリングにも成功している。この手法は、細胞アッセイの予測精度向上に繋がるものと期待される。\n研究成果の概要（英文）： We investigated towards the development of label-free cell-based bioassay systems with oxygen-independent electrochemical biosensors. Based on the electrochemical responses obtained here, hepatocytes cultured under an aerobic culture method showed physiologically relevant behaviors of glucose metabolism and gluconeogenesis in the periportal region of liver tissues. In addition, released histamine from a basophilic leukemia cell line, RBL-2H3, was successfully monitored after immunostimulation. The present systems will enable to improve the prediction of human responses.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C); 研究期間：2019～2021; 課題番号：19K12751; 研究分野：生物工学; 科研費の分化・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究協力者"},{"subitem_text_value":"研究協力者"},{"subitem_text_value":"研究協力者"},{"subitem_text_value":"研究協力者"},{"subitem_text_value":"研究協力者"},{"subitem_text_value":"研究協力者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Komori, Kikuo"},{"subitem_text_language":"en","subitem_text_value":"Sakai, Yasuyuki"},{"subitem_text_language":"en","subitem_text_value":"Kimura, Hiroshi"},{"subitem_text_language":"en","subitem_text_value":"Tixier Mita, Agnes"},{"subitem_text_language":"en","subitem_text_value":"Komatsu, Yuto"},{"subitem_text_language":"en","subitem_text_value":"Usui, Masataka"},{"subitem_text_language":"en","subitem_text_value":"Zhu, Dongchen"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学工学部; 准教授"},{"subitem_text_value":"東京大学工学系研究科; 教授"},{"subitem_text_value":"東海大学マイクロ・ナノ研究開発センター; 教授"},{"subitem_text_value":"東京大学生産技術研究所; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小森, 喜久夫"},{"creatorName":"コモリ, キクオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{"nameIdentifierURI":""}]},{"creatorNames":[{"creatorName":"酒井, 康行"},{"creatorName":"サカイ, ヤスユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{"nameIdentifierURI":""}]},{"creatorNames":[{"creatorName":"木村, 啓志"},{"creatorName":"キムラ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{"nameIdentifierURI":""}]},{"creatorNames":[{"creatorName":"ティクシエ三田, アニエス"},{"creatorName":"ティクシエ, ミタ  アニエス","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{"nameIdentifierURI":""}]},{"creatorNames":[{"creatorName":"小松, 唯人"},{"creatorName":"コマツ, ユウト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"碓井, 政貴"},{"creatorName":"ウスイ, マサタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"朱, 東晨"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-12-05"}],"displaytype":"detail","filename":"19K12751seika.pdf","filesize":[{"value":"226.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"19K12751seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/23244/files/19K12751seika.pdf"},"version_id":"39f60b5a-6663-4f2a-803d-f73ab09846b8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"電気化学センサ","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞アッセイ","subitem_subject_scheme":"Other"},{"subitem_subject":"カーボンナノ材料","subitem_subject_scheme":"Other"},{"subitem_subject":"生体模倣システム","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ダイナミック細胞応答を観測可能な統合化インビトロシステム構築","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ダイナミック細胞応答を観測可能な統合化インビトロシステム構築","subitem_title_language":"ja"},{"subitem_title":"Development of integrated in vitro monitoring systems for dynamic cell responses","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4819"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-12-05"},"publish_date":"2022-12-05","publish_status":"0","recid":"23244","relation_version_is_last":true,"title":["ダイナミック細胞応答を観測可能な統合化インビトロシステム構築"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-01T00:38:52.057180+00:00"}