{"created":"2023-06-20T16:50:39.617210+00:00","id":23225,"links":{},"metadata":{"_buckets":{"deposit":"8ca47f69-d68a-492f-ace2-3eb28c306295"},"_deposit":{"created_by":3,"id":"23225","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"23225"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00023225","sets":["14:923:1477:4818"]},"author_link":["41136"],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"72","bibliographicPageStart":"63","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[抄録] 血管内皮の酸化LDL受容体として同定されたレクチン様酸化LDL受容体-1(LOX-1)の軟骨細胞での発現が知られ，酸化低比重リポ蛋白(酸化LDL)と軟骨変性との関連が注目されている．一方，血管内皮増殖因子VEGFとその受容体が軟骨細胞にも発現しMMPs産生を介して軟骨変性に関与することが示されている．本研究の目的は軟骨細胞における酸化LDL・LOX-1結合によるVEGF発現，さらにVEGF発現における核内転写因子ペロキシソーム増殖因子応答性受容体PPAR-γ活性化の関与を検討することである．まず，酸化LDL添加によるVEGF発現を検討し，さらに抗LOX-1抗体による抑制実験を行った．その結果，mRNA，蛋白レベルにおいて，酸化LDL添加によりVEGF発現の亢進が認められ，これらは抗LOX-1抗体によって抑制された．さらに酸化LDL添加によるPPAR-γの活性化・発現を検討し，PPAR-γのAgonist， Antagonistを用いてVEGF発現を検討した．その結果，酸化LDL・LOX-1結合はPPAR-γ活性化・発現亢進を介してVEGF発現を亢進していることが明らかになった．酸化LDLによる軟骨変性のメカニズムの一つに，PPAR-γ活性化を介したVEGF発現亢進があることが示唆された．","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部整形外科学教室"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"金田, 宗也"}],"nameIdentifiers":[{"nameIdentifier":"41136","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-04"}],"displaytype":"detail","filename":"AN00063584-20051225-0063.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00063584-20051225-0063.pdf","url":"https://kindai.repo.nii.ac.jp/record/23225/files/AN00063584-20051225-0063.pdf"},"version_id":"e55096a5-3199-4906-b9fa-1f776e508b3a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chondrocyte","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oxidized LDL","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"LOX-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PPAR-γ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cartilage metabolism","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈原著〉牛培養軟骨細胞における血管内皮増殖因子(VEGF)の発現亢進とペロキシソーム増殖因子応答性受容体(PPAR-γ)の活性化：酸化低比重リポ蛋白(LDL)とレクチン様酸化LDL受容体-1(LOX-1)の関与について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈原著〉牛培養軟骨細胞における血管内皮増殖因子(VEGF)の発現亢進とペロキシソーム増殖因子応答性受容体(PPAR-γ)の活性化：酸化低比重リポ蛋白(LDL)とレクチン様酸化LDL受容体-1(LOX-1)の関与について"}]},"item_type_id":"2","owner":"3","path":["4818"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-04"},"publish_date":"2022-10-04","publish_status":"0","recid":"23225","relation_version_is_last":true,"title":["〈原著〉牛培養軟骨細胞における血管内皮増殖因子(VEGF)の発現亢進とペロキシソーム増殖因子応答性受容体(PPAR-γ)の活性化：酸化低比重リポ蛋白(LDL)とレクチン様酸化LDL受容体-1(LOX-1)の関与について"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T19:57:32.397676+00:00"}