{"created":"2023-06-20T16:49:59.891428+00:00","id":22386,"links":{},"metadata":{"_buckets":{"deposit":"87c45f0c-1dd3-4f29-9cfb-8b4e92945464"},"_deposit":{"created_by":3,"id":"22386","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"22386"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00022386","sets":["14:2667:4710"]},"author_link":["3389","3859","43012"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2020)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K06021/ | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K06021/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：絶滅危惧動物であるトゲネズミの幹細胞の樹立を目的として、異種間顕微授精を用いた雄性2倍体胚由来ES細胞の樹立について検討した。ラットおよびモリアカネズミ精子をマウス除核卵子に顕微注入したが胚発生は停止した。そこで、マウス卵子の異種精子の受容能についてハイブリッド胚による解析を行った。その結果、モリアカネズミ精子では雄性前核の形成が遅延すること、先体酵素の除去処理により異種雄性前核の形成遅延が改善されることが明らかとなった。アマミトゲネズミ－マウス異種間体細胞核移植胚の作製とES細胞の樹立を試みた。しかし、胚盤胞への発生率は極めて低く、発生した胚盤胞からもntES細胞の樹立には至らなかった。研究成果の概要（英文）：For the purpose of establishing ES cells derived from androgenic diploid embryos produced by interspecific sperm injection, either rat or wood mouse (Apodemus sylvaticus) sperm were microinjected into mouse enucleated oocytes, but embryonic development was arrested. Therefore, in order to verify the acceptance of heterologous sperm in mouse oocytes, hybrid embryos were analyzed. As a result, the formation of the male pronucleus was delayed in the wood mouse sperm, and the delay in the formation of the heterologous male pronucleus was improved by the removal of the acrosomal enzyme or the destabilization of the sperm cell membrane. Due to COVID-19, original research plan was changed to establish ES cells from the embryos of Ryukyu spiny rat using interspecific somatic cell nuclear transfer. However, its developmental ability to the blastocysts was extremely low, and ntES cells could not be established.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2018～2020;   課題番号：18K06021;   研究分野：生殖生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Mitani, Tasuku"},{"subitem_text_language":"en","subitem_text_value":"Tanabe, Hideyuki"},{"subitem_text_language":"en","subitem_text_value":"Okamura, Daiji"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 教授"},{"subitem_text_value":"総合研究大学院大学先導科学研究科; 准教授"},{"subitem_text_value":"近畿大学農学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"　"},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三谷, 匡"},{"creatorName":"ミタニ, タスク","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"田辺, 秀之"},{"creatorName":"タナベ, ヒデユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"岡村, 大治"},{"creatorName":"オカムラ, ダイジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-07"}],"displaytype":"detail","filename":"18K06021seika.pdf","filesize":[{"value":"107.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"18K06021seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/22386/files/18K06021seika.pdf"},"version_id":"a5eabdfa-57a1-4371-9f67-4477aaae4405"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"雄性2倍体胚","subitem_subject_scheme":"Other"},{"subitem_subject":"ハイブリッド胚","subitem_subject_scheme":"Other"},{"subitem_subject":"ES細胞","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"異種間顕微授精によるトゲネズミ雄性2倍体胚由来ES細胞の樹立と配偶子形成の誘導","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"異種間顕微授精によるトゲネズミ雄性2倍体胚由来ES細胞の樹立と配偶子形成の誘導"},{"subitem_title":"Establishment of ES cells derived from androgenic diploid embryos produced by interspecific sperm injection and induction of gametogenesis","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4710"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-07"},"publish_date":"2022-03-07","publish_status":"0","recid":"22386","relation_version_is_last":true,"title":["異種間顕微授精によるトゲネズミ雄性2倍体胚由来ES細胞の樹立と配偶子形成の誘導"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T19:46:36.661702+00:00"}