{"created":"2023-06-20T16:49:59.588640+00:00","id":22379,"links":{},"metadata":{"_buckets":{"deposit":"806f3cbe-5fcc-4551-b97e-8e1c6a78356e"},"_deposit":{"created_by":3,"id":"22379","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"22379"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00022379","sets":["14:2667:4710"]},"author_link":["31971","33643"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2020)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K00421/ | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K00421/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：アルツハイマー病の初期分子過程ではまずアミロイド前駆体タンパク質（APP）がベータ切断酵素によって切断される。その後ガンマ切断酵素によって切断され、アミロイドβペプチドが産生される。本研究では、APPとベータ切断酵素との相互作用過程に注目した。まず、ベータ切断酵素の膜貫通部位の構造が解かれていなかったので、レプリカ交換分子動力学法（REMD）を用い立体構造を予測した。その構造を元にして粗視化モデルシミュレーションを行い、APPの膜貫通部位とベータ切断酵素の膜貫通部位の相互作用ポイントを明らかにした。更にα切断酵素の膜貫通部位の構造予測をし、α切断酵素とAPPの相互作用も明らかにした。研究成果の概要（英文）： In the early stage of Alzheimer's disease, amyloid precursor protein (APP) is cleaved by beta-secretase, then it is cleaved by gamma-secretase, and Amyloid beta-peptide is produced. In this research, we have focused on the interaction between APP and beta-secretase in the membrane. The transmembrane (TM) regions of beta-secretase have not been solved the tertiary structure. Thus, we performed the replica exchange molecular dynamics (REMD) simulation to predict the tertiary structure of the TM domain of beta-secretase. Then, we performed coarse-grained model molecular dynamics simulations of the TM domain of beta-secretase and TM domain of APP in the bio-membrane to reveal the interaction mechanism between APP and beta-secretase. We also cleared the interaction mechanism of APP and alpha-secretase.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2017～2020;   課題番号：17K00421;   研究分野：分子シミュレーション・医療情報;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究協力者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Miyashita, Naoyuki"},{"subitem_text_language":"en","subitem_text_value":"Yonezawa, Yasushige"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 准教授"},{"subitem_text_value":"近畿大学先端技術総合研究所; 教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"宮下, 尚之"},{"creatorName":"ミヤシタ, ナオユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"米澤, 康滋"},{"creatorName":"ヨネザワ, ヤスシゲ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-07"}],"displaytype":"detail","filename":"17K00421seika.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"17K00421seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/22379/files/17K00421seika.pdf"},"version_id":"ef070ab2-0793-4017-b1e2-6230b338ecf3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"アルツハイマー病","subitem_subject_scheme":"Other"},{"subitem_subject":"β切断酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"α切断酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"生体膜","subitem_subject_scheme":"Other"},{"subitem_subject":"ラフト","subitem_subject_scheme":"Other"},{"subitem_subject":"相互作用","subitem_subject_scheme":"Other"},{"subitem_subject":"分子動力学シミュレーション","subitem_subject_scheme":"Other"},{"subitem_subject":"構造予測","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"分子シミュレーションによるアルツハイマー病初期分子機構への生体膜環境の影響解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"分子シミュレーションによるアルツハイマー病初期分子機構への生体膜環境の影響解析"},{"subitem_title":"Molecular Simulation Study of the Bio-membrane of the neuron in the early stage of Alzheimer's disease","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4710"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-07"},"publish_date":"2022-03-07","publish_status":"0","recid":"22379","relation_version_is_last":true,"title":["分子シミュレーションによるアルツハイマー病初期分子機構への生体膜環境の影響解析"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T19:46:21.515187+00:00"}