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〈論文：Paper〉 Real-time monitoring of B16 melanoma cell viability by the firefly luciferase-based bioluminescence
https://kindai.repo.nii.ac.jp/records/22266
https://kindai.repo.nii.ac.jp/records/22266ce57dacb-b467-48b8-b0d2-112185d1d443
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN10074306-20210531-0005.pdf (1.9 MB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-01-21 | |||||
| タイトル | ||||||
| タイトル | 〈論文：Paper〉 Real-time monitoring of B16 melanoma cell viability by the firefly luciferase-based bioluminescence | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Firefly luciferase, melanoma, cytotoxicity, lymphocytes | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者(英) | ||||||
| 言語 | en | |||||
| 値 | Nishizawa,Hinako | |||||
| 著者(英) | ||||||
| 言語 | en | |||||
| 値 | Kurowarabe,Kaoru | |||||
| 著者(英) | ||||||
| 言語 | en | |||||
| 値 | Hayashi,Ryota | |||||
| 著者(英) | ||||||
| 言語 | en | |||||
| 値 | Hayasaka,Haruko | |||||
| 著者 所属 | ||||||
| 値 | 近畿大学 | |||||
| 著者所属(翻訳) | ||||||
| 値 | Laboratory of Immune Molecular Function, Faculty of Science & Engineering | |||||
| 著者所属(翻訳) | ||||||
| 値 | Depertment of Science, Graduate School of Science and Engineering | |||||
| 著者所属(翻訳) | ||||||
| 値 | Depertment of Science, Graduate School of Science and Engineering | |||||
| 著者所属(翻訳) | ||||||
| 値 | Laboratory of Immune Molecular Function, Faculty of Science & Engineering: Depertment of Science, Graduate School of Science and Engineering: Research Institute for Science and Technology, Kindai University | |||||
| 版 | ||||||
| 出版タイプ | NA | |||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||
| 出版者 名前 | ||||||
| 出版者 | 近畿大学理工学総合研究所 | |||||
| 書誌情報 |
理工学総合研究所研究報告 en : Annual Reports by Science and Technology Research Institute 号 32, p. 5-11, 発行日 2021-05-31 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09162054 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | [Abstract] Various cytotoxicity assays have been primarily established by parameters associated with plasma membrane permeability. However, current methods are not suitable for cell viability measurement in mixed cell cultures, for example, in an analysis of cell-mediated cytotoxicity between responder cells (such as cytotoxic T cells) and the target cells (tumor cells). In this study, we attempted to establish an experimental system using firefly luciferase bioluminescence, which is useful for quantification and real-time monitoring of target cell viability. We transfected B16F10 melanoma cells with the expression plasmid containing the firefly luciferase gene and monitored bioluminescent signals associated with apoptotic cell death induced by calcium ionophore A23187 or an aminonucleoside antibiotic, puromycin. With A23187 or puromycin treatment, we observed a remarkable, concentration- and time-dependent decrease in the luminescence value. When B16F10 melanoma cells were co-cultured with stimulated splenocytes, the luminescence value gradually decreased, whereas no splenocytes, the luminescence value gradually increased for about 30 h. These results imply that the activated responder cells affected the target B16F10 viability, by inducing apoptotic cell death. We propose that the firefly luciferase-based reporter is a useful experimental system for quantifying cellmediated cytotoxicity between the responder and the target cells. | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
