{"created":"2023-06-20T16:49:41.318856+00:00","id":21948,"links":{},"metadata":{"_buckets":{"deposit":"37fdfca3-d7b0-4dc0-b5a5-1c8c21e8e348"},"_deposit":{"created_by":3,"id":"21948","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"21948"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00021948","sets":["14:923:1809:4692"]},"author_link":["37851","37852","37853","37854","37855","37856","37857","37858"],"control_number":"21948","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"32","bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"かやのもり：近畿大学産業理工学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Reports of Faculty of Humanity-Oriented Science and Engineering, Kindai University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the present study, quantitative analysis of single nucleotide mutated genes by allele specific real time PCR using phosphorothioate primers and DNA polymerases is described. DNA polymerases involve taq DNA polymerases （TaKaRa Taq PolymeraseTM and HiDi PolymeraseTM） which do not have 3’-exonuclease activity and HiFi taq DNA polymerase （TaKaRa Ex Taq PolymeraseTM） which has 3’-exonuclease activity, namely proofreading activity. Consequently, accuracy of discrimination of single base mutation was not so high in the PCR using TaKaRa Taq PolymeraseTM and TaKaRa Ex Taq PolymeraseTM. On the other hand, HiDi PolymeraseTM showed high accuracy of discrimination of single base mutation between KRAS wt and KRAS G12D model DNA templates.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15100/0000021948","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学産業理工学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13495801","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"琉球大学熱帯生物圏研究センター環境生命情報学分野; 博士研究員"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 教授"},{"subitem_text_value":"近畿大学農学部生物機能科学科; 教授"},{"subitem_text_value":"近畿大学農学部生物機能科学科; 助教"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 教授"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"園川, 舞華","creatorNameLang":"ja"},{"creatorName":"ソノカワ, マイカ","creatorNameLang":"ja-Kana"},{"creatorName":"Sonokawa, Maika","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"久野, 雅明","creatorNameLang":"ja"},{"creatorName":"ヒサノ, マサアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Hisano, Masaaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤田, 崇史","creatorNameLang":"ja"},{"creatorName":"フジタ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujita,Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"塩浜, 康雄","creatorNameLang":"ja"},{"creatorName":"シオハマ, ヤスオ","creatorNameLang":"ja-Kana"},{"creatorName":"Shiohama, Yasuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"神武, 洋二郎","creatorNameLang":"ja"},{"creatorName":"コウタケ, ヨウジロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kotake, Yojiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"北山, 隆","creatorNameLang":"ja"},{"creatorName":"キタヤマ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Kitayama, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"柏﨑, 玄伍","creatorNameLang":"ja"},{"creatorName":"カシワザキ, ゲンゴ","creatorNameLang":"ja-Kana"},{"creatorName":"Kashiwazaki, Gengo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤井, 政幸","creatorNameLang":"ja"},{"creatorName":"フジイ, マサユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-25"}],"displaytype":"detail","filename":"AA12013209-20210630-0001.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA12013209-20210630-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/21948/files/AA12013209-20210630-0001.pdf"},"version_id":"ff64b502-d96e-412c-826e-1f9b4c3318e6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Quantitaive PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Single Base Mutation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"KRAS wt","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"KRAS G 12D","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphorothioate Primer","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈論文・報告〉ホスホロチオエート核酸をプライマーとする一塩基変異識別定量PCR法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈論文・報告〉ホスホロチオエート核酸をプライマーとする一塩基変異識別定量PCR法","subitem_title_language":"ja"},{"subitem_title":"〈PAPERS and REPORTS〉Quantitative PCR Discriminating Single Base Mutation Using Phosphorothioate Primers","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["4692"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-08-25"},"publish_date":"2021-08-25","publish_status":"0","recid":"21948","relation_version_is_last":true,"title":["〈論文・報告〉ホスホロチオエート核酸をプライマーとする一塩基変異識別定量PCR法"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-14T07:53:10.653541+00:00"}