@article{oai:kindai.repo.nii.ac.jp:00021948,
 author = {園川, 舞華 and Sonokawa, Maika and 久野, 雅明 and Hisano, Masaaki and 藤田, 崇史 and Fujita,Takashi and 塩浜, 康雄 and Shiohama, Yasuo and 神武, 洋二郎 and Kotake, Yojiro and 北山, 隆 and Kitayama, Takashi and 柏﨑, 玄伍 and Kashiwazaki, Gengo and 藤井, 政幸 and Fujii, Masayuki},
 issue = {32},
 journal = {かやのもり：近畿大学産業理工学部研究報告, Reports of Faculty of Humanity-Oriented Science and Engineering, Kindai University},
 month = {Jun},
 note = {In the present study, quantitative analysis of single nucleotide mutated genes by allele specific real time PCR using phosphorothioate primers and DNA polymerases is described. DNA polymerases involve taq DNA polymerases （TaKaRa Taq PolymeraseTM and HiDi PolymeraseTM） which do not have 3’-exonuclease activity and HiFi taq DNA polymerase （TaKaRa Ex Taq PolymeraseTM） which has 3’-exonuclease activity, namely proofreading activity. Consequently, accuracy of discrimination of single base mutation was not so high in the PCR using TaKaRa Taq PolymeraseTM and TaKaRa Ex Taq PolymeraseTM. On the other hand, HiDi PolymeraseTM showed high accuracy of discrimination of single base mutation between KRAS wt and KRAS G12D model DNA templates., application/pdf},
 pages = {1--8},
 title = {〈論文・報告〉ホスホロチオエート核酸をプライマーとする一塩基変異識別定量PCR法},
 year = {2021},
 yomi = {ソノカワ, マイカ and ヒサノ, マサアキ and フジタ, タカシ and シオハマ, ヤスオ and コウタケ, ヨウジロウ and キタヤマ, タカシ and カシワザキ, ゲンゴ and フジイ, マサユキ}
}