{"created":"2023-06-20T16:49:19.040553+00:00","id":21512,"links":{},"metadata":{"_buckets":{"deposit":"9139074a-dcb8-4158-98d4-34c858dcef22"},"_deposit":{"created_by":3,"id":"21512","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"21512"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00021512","sets":["14:2667:4613"]},"author_link":["43184"],"control_number":"21512","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"9","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2019)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K01398/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：骨の90％は，リン酸カルシウムとＩ型コラーゲンでできている．コラーゲンは架橋することで物理的強度を高めていることから，その抽出回収は容易ではなかった．本課題では，骨組織から独自の方法で骨コラーゲンを回収する方法を確立し，その機能を骨関連細胞で検証することを目的とした．その結果，温和な条件で効率的に骨由来Ｉ型コラーゲンを再現性良く精製することに成功した．LC-MSで骨コラーゲンの水酸基などの翻訳後修飾を同定した．また骨コラーゲンを培養足場として活用した結果，間葉系幹細胞の骨芽細胞への分化誘導が短期間に進むことを確認した．これまでにない骨コラーゲンの再生医療への活用が期待される．研究成果の概要（英文）： 90% of bone is made of calcium phosphate and type I collagen. Since collagen has increased physical strength by cross-linking, its extraction and recovery was not easy. The purpose of this study was to establish a unique method for recovering bone collagen from bone tissue and to verify its function in bone-related cells. As a result, we succeeded in efficiently and efficiently purifying bone-derived type I collagen under mild conditions. Post-translational modifications such as hydroxyl groups of bone collagen were identified by LC-MS. As a result of utilizing bone collagen as a culture scaffold, it was confirmed that the induction of differentiation of mesenchymal stem cells into osteoblasts proceeded in a short period of time. It is expected that bone collagen will be utilized in regenerative medicine like never before.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2017～2019;   課題番号：17K01398;   研究分野：バイオマテリアル;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"MORIMOTO, KOICHI"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森本, 康一"},{"creatorName":"モリモト, コウイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-15"}],"displaytype":"detail","filename":"17K01398seika.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"17K01398seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/21512/files/17K01398seika.pdf"},"version_id":"2b8811e5-2e6a-47a5-96f5-af543b12d858"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"コラーゲン","subitem_subject_scheme":"Other"},{"subitem_subject":"骨組織","subitem_subject_scheme":"Other"},{"subitem_subject":"翻訳後修飾","subitem_subject_scheme":"Other"},{"subitem_subject":"間葉系幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"培養足場","subitem_subject_scheme":"Other"},{"subitem_subject":"qRT-PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"骨芽細胞分化誘導","subitem_subject_scheme":"Other"},{"subitem_subject":"軟骨細胞分化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"骨コラーゲンの新規精製法の確立と遺伝子発現量比較による骨関連細胞の機能解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"骨コラーゲンの新規精製法の確立と遺伝子発現量比較による骨関連細胞の機能解析","subitem_title_language":"ja"},{"subitem_title":"Establishment of new purification method for bone collagen and functional analysis of bone-related cells by comparing gene expression levels","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4613"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-03-15"},"publish_date":"2021-03-15","publish_status":"0","recid":"21512","relation_version_is_last":true,"title":["骨コラーゲンの新規精製法の確立と遺伝子発現量比較による骨関連細胞の機能解析"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-14T06:49:52.069740+00:00"}