{"created":"2023-06-20T16:49:18.018182+00:00","id":21489,"links":{},"metadata":{"_buckets":{"deposit":"da83359c-bab9-4d79-a2b3-2264580cc763"},"_deposit":{"created_by":3,"id":"21489","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"21489"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00021489","sets":["14:2667:4613"]},"author_link":["4060"],"control_number":"21489","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2019)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K08680/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：緑内障や腸閉塞などでは内圧上昇によって細胞変性が生じるが、その分子機序は未解明である。この現象を検証するため、独自に考案した培養系を樹立した。円柱形の形態を示す上皮細胞では、圧上昇に随伴して細胞増殖が抑制された。細胞は扁平化し体積が増加したため、細胞骨格であるアクチン線維の分布が疎になることを見出した。また、Hippo経路の主要分子であるYAPのリン酸化が亢進し、細胞質局在量が増加していた。以上より、円柱形の上皮細胞ではその増殖に細胞形態と細胞骨格とが深く関わることが明らかになった。内圧上昇はこの連関及びHippo経路に直接的に作用し上皮変性を惹起すると考えられた。研究成果の概要（英文）：Intraluminal pressure elevation can be pathogenic to mucosal epithelia even at a few tens cmH2O, but its effect on cell growth has not been examined intensively. A two-chamber culture system was modified to make cells receive water pressure. The cells with columnar epithelial morphology were growth-suppressed in a manner dependent on water pressure. Phalloidin staining revealed that a pressure load vertically flattened and laterally widened columnar epithelial cells and made actin fiber distribution sparse. In addition, a pressure load enhanced serine-127 phosphorylation and cytoplasmic retention of YAP, the major constituent of the Hippo pathway, suggesting that Hippo pathway was involved in the pressure-induced cell growth suppression. These results provide evidence that cell morphology and the cytoskeleton are closely linked to cell growth. Pathological intraluminal pressure elevation may cause mucosal degeneration by acting directly on this linkage and the Hippo pathway.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2017～2019;   課題番号：17K08680;   研究分野：実験病理;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Hagiyama, Man"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 助教"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"萩山, 満"},{"creatorName":"ハギヤマ, マン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-15"}],"displaytype":"detail","filename":"17K08680seika.pdf","filesize":[{"value":"503.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"17K08680seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/21489/files/17K08680seika.pdf"},"version_id":"82085269-2c28-49aa-a2a0-5d6cc30e9a96"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"細胞変性","subitem_subject_scheme":"Other"},{"subitem_subject":"内圧上昇","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"圧付加による神経変性：水柱下培養装置を用いた分子基盤の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"圧付加による神経変性：水柱下培養装置を用いた分子基盤の解析","subitem_title_language":"ja"},{"subitem_title":"Nerve degeneration by modest static pressure: a new water-pressure-loadable culture system","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4613"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-03-15"},"publish_date":"2021-03-15","publish_status":"0","recid":"21489","relation_version_is_last":true,"title":["圧付加による神経変性：水柱下培養装置を用いた分子基盤の解析"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-14T02:16:39.090713+00:00"}