{"created":"2023-06-20T16:49:17.084973+00:00","id":21468,"links":{},"metadata":{"_buckets":{"deposit":"ab460725-b0aa-472d-9df2-a2888b14dcc0"},"_deposit":{"created_by":3,"id":"21468","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"21468"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00021468","sets":["14:2667:4613"]},"author_link":["31833"],"control_number":"21468","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"9","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2019)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K07736/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：イオン液体は、生体触媒の新たな反応溶媒として注目される。しかし親水性・疎水性イオン液体による細菌の生育阻害が問題であり、解決策が模索されている。本研究では、独自に単離したイオン液体耐性菌Bacillus sp. CMW1を用いて、イオン液体耐性に関わる遺伝子を解明して、イオン液体耐性に優れた細菌宿主の開発を目的とした。その結果、本菌株のイオン液体耐性には、多剤排出トランスポーター遺伝子が関与することを見いだした。本遺伝子をBrevibacillus 属細菌に導入して、イオン液体耐性に優れた細菌宿主を構築できた。CMW1株の形質転換系を構築して、疎水性イオン液体耐性に優れた変異株を取得した。研究成果の概要（英文） ；Ionic liquids have physical properties for use as reaction solvents. To solve the problems of growth inhibition of microorganisms by ionic liquids, we isolated the ionic liquid-tolerant Bacillus sp. CMW1. The first objective of this study is to identify the gene which are responsible for ionic-liquid tolerant. The second objective is to develop the novel bacterial\\\nhost which exhibits tolerance to ionic liquids. Consequently, I identified MFS transporter gene from strain CMW1 and showed that the gene is\\\nresponsible for an ionic-liquid tolerant. The gene of CMW1 was transformed into Brevibacillus sp. bacterium and the recombinant strain could grow in the presence of an ionic liquid. Using tetracycline, pHY300PLK was successfully electroporated into strain CMW1 with the efficiency. I\\\nobtained the mutants which were able to grow in the presence of hydrophobic ionic liquids. The results will provide a basis for the future work in biocatalysis with various ionic liquids in industry.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2017～2019;   課題番号：17K07736;   研究分野：応用微生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Kurata, Atsushi"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"倉田, 淳志"},{"creatorName":"クラタ, アツシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"31833","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10416000","nameIdentifierScheme":"NRID","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?kw=10416000"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-15"}],"displaytype":"detail","filename":"17K07736seika.pdf","filesize":[{"value":"294.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"17K07736seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/21468/files/17K07736seika.pdf"},"version_id":"fdedb3a7-0f96-4ec7-8079-47fa206e960d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"イオン液体","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacillus 属細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"イオン液体耐性細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"多剤排出トランスポーター","subitem_subject_scheme":"Other"},{"subitem_subject":"浸透圧ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"ゲノム解析","subitem_subject_scheme":"Other"},{"subitem_subject":"イオン液体耐性酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA-seq","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"イオン液体耐性菌の耐性機構の解明と新奇微生物プラットフォームの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イオン液体耐性菌の耐性機構の解明と新奇微生物プラットフォームの開発","subitem_title_language":"ja"},{"subitem_title":"Elucidation of an ionic-liquid resistance mechanism of an ionic-liquid resistant bacterium and development of a novel microbial platform","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4613"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-03-15"},"publish_date":"2021-03-15","publish_status":"0","recid":"21468","relation_version_is_last":true,"title":["イオン液体耐性菌の耐性機構の解明と新奇微生物プラットフォームの開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-13T07:16:16.698243+00:00"}