{"created":"2023-06-20T16:49:14.308614+00:00","id":21405,"links":{},"metadata":{"_buckets":{"deposit":"2e49187b-da2e-4b40-a428-2aa01f35c103"},"_deposit":{"created_by":3,"id":"21405","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"21405"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00021405","sets":["14:2667:4613"]},"author_link":["30609","3607","3835"],"control_number":"21405","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"16","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2019)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K01925/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：我々は、これまでに、ペプチドホルモン前駆体のＸ線結晶構造解析および部位特異的変異体の立体構造形成反応の詳細な分析から、ペプチドホルモン前駆体蛋白質の立体構造形成が、成熟体であるペプチドホルモンの局部的な生理活性部位の立体構造の安定化と直接関係することを明らかにした。そこで、その立体構造形成を最終的に制御する部位について焦点を当て、その2次構造変化を利用した立体構造制御機構について、分子進化の概念に基づき検討を行った。NMRおよびX線結晶構造解析によって得られた最終物とフォールディング反応中間体の立体構造を比較することに成功し、新たな生理活性分子の構築に成功した。研究成果の概要（英文）：To investigate how peptide hormones develop their biological activity on molecular evolution, the folding mechanism of the precursor proteins, such as prouroguanylin and pro-ANP were investigated. Structural analyses of the proteins by means of NMR and X-ray crystallography including their folding intermediates suggested that the secondary structural shift at the hinge region of the processing sites maintains the correct conformation of the final folding products during the molecular evolution. In addition,the propeptide region was fused to de novo designed peptides to regulate the bioactive conformation, resulting in the stabilization of the correct conformation of the de novo peptide. Thus, we successfully obtained the bioactive de novo peptides.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2016～2019;   課題番号：16K01925;   研究分野：タンパク質・ペプチド科学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"連携研究者"},{"subitem_text_value":"連携研究者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Hidaka, Yuji"},{"subitem_text_language":"en","subitem_text_value":"Yamaguchi, Hiroshi"},{"subitem_text_language":"en","subitem_text_value":"Shimamoto, Shigeru"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学理工学部; 教授"},{"subitem_text_value":"関西学院大学理工学部; 教授"},{"subitem_text_value":"近畿大学理工学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":""},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"日高, 雄二"},{"creatorName":"ヒダカ, ユウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"山口, 宏"},{"creatorName":"ヤマグチ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"島本, 茂"},{"creatorName":"シマモト, シゲル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-15"}],"displaytype":"detail","filename":"16K01925seika.pdf","filesize":[{"value":"114.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"16K01925seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/21405/files/16K01925seika.pdf"},"version_id":"bf8be8f9-d284-40a9-a962-30f5357f799b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"前駆体","subitem_subject_scheme":"Other"},{"subitem_subject":"フォールディング","subitem_subject_scheme":"Other"},{"subitem_subject":"ペプチドホルモン","subitem_subject_scheme":"Other"},{"subitem_subject":"ジスルフィド","subitem_subject_scheme":"Other"},{"subitem_subject":"分子進化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"前駆体蛋白質の分子進化における品質管理機構の解明と分子設計への応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"前駆体蛋白質の分子進化における品質管理機構の解明と分子設計への応用","subitem_title_language":"ja"},{"subitem_title":"Molecular evolution of precursor protein of peptide hormone and its application for molecular modeling","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4613"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-03-15"},"publish_date":"2021-03-15","publish_status":"0","recid":"21405","relation_version_is_last":true,"title":["前駆体蛋白質の分子進化における品質管理機構の解明と分子設計への応用"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-12T07:54:48.708452+00:00"}