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角膜実質細胞によるコラ-ゲン分解の調節機序について
https://kindai.repo.nii.ac.jp/records/2138
https://kindai.repo.nii.ac.jp/records/213857902260-1ede-4bdc-b793-caf47d59be7d
| 名前 / ファイル | ライセンス | アクション |
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Thesis_Mek0225-19940324-0001.pdf (1.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2014-01-20 | |||||||||
| タイトル | ||||||||||
| タイトル | 角膜実質細胞によるコラ-ゲン分解の調節機序について | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | Regulatory mechanism of collagen degradation by keratocytes | |||||||||
| 著者 |
岡本, 純之助
× 岡本, 純之助
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | collagen degradation, keratocytes, collagenase, interleukin 1 (IL- 1), interleukin 1 receptor antagonist (IL- 1ra), cytochalasin B, chloroquine | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||||
| 資源タイプ | thesis | |||||||||
| 著者(英) | ||||||||||
| en | ||||||||||
| Okamoto, Junnosuke | ||||||||||
| 著者 所属 | ||||||||||
| 近畿大学大学院 医学研究科 外科学系専攻 | ||||||||||
| 著者所属(翻訳) | ||||||||||
| Department of Ophthalmology, Kinki University School of Medicine | ||||||||||
| 版 | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学 | |||||||||
| 書誌情報 |
博士学位論文 en : Thesis or Dissertation p. 1-26, 発行日 1994-03-01 |
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | [ABSTRACT] Excessive degradation of stromal collagen fibrils occurs in corneal ulceration. Keratocytes (corneal fibroblasts) seem to play a primary role in collagen degradation in corneal ulceration. However, the mechanisms of collagen degradation in corneal ulceration have not been fully understood. Tounderstand the pathobiology of corneal ulceration, I developed a new in-vitro model in which keratocytes were cultured in a collagen matrix threedimensionally. Using this model, I searched for any factors which might regulate collagen degradation by keratocytes. Collagen degradation wasmeasured by the amount of hydroxyproline in the hydrolysate. Collagen degradation by keratocytes depended on the number of cells, the concentration of collagen and the period of incubation. Among various cytokines and growth factors such as epidermal growth factor ( EGF ), fibroblast growth factor ( FGF ) , insulin-like growth factor 1 ( IGF-1) , interleukin 1 (IL- 1) and interleukin 6 (IL- 6), only IL- 1 was found to enhance collagen degradation by keratocytes in a dose dependent manner. Interleukin 1 receptor antagonist (IL- 1 ra) antagonized the stimulatory effect of IL-1 on collagen degradation by keratocytes. IL- 1 also stimulated collagenase synthesis by keratocytes. When phagocytic activity or intracellular lysosomal enzyme activities were inhibited by the addition of cytochalasin B or chloroquine, collagen degradation showed a decrease in the presence of IL- 1 . These findings suggested that both extracellular and intracellular collagen degradation played important roles in the stimulatory effect of IL- 1 on collagen degradation by keratocytes. | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 学位の種類：医学 学位授与年月日：1994/3/24 指導： 大鳥, 利文 教授(Director: Prof. Otori, Toshifumi) 報告番号：甲第278号 学内授与番号：医甲225 NDL書誌ID：000000278319 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
