WEKO3
アイテム
角膜上皮創傷治癒におけるフィブロネクチン受容体(インテグリン)の役割について
https://kindai.repo.nii.ac.jp/records/2124
https://kindai.repo.nii.ac.jp/records/212423a0eeb5-adb2-43f1-b818-5be3b9facd96
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Thesis_Mek0168-19920319-0001.pdf (1.5 MB)
|
|
| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2011-12-09 | |||||||||
| タイトル | ||||||||||
| タイトル | 角膜上皮創傷治癒におけるフィブロネクチン受容体(インテグリン)の役割について | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | Fibronectin receptors (integrin) in corneal epithelial wound healing | |||||||||
| 著者 |
村上, 純子
× 村上, 純子
|
|||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | fibronectin receptor, fibronectin, wound healing, cornea, epithelium | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||||
| 資源タイプ | thesis | |||||||||
| 著者(英) | ||||||||||
| en | ||||||||||
| Murakami, Junko | ||||||||||
| 著者 所属 | ||||||||||
| 近畿大学大学院 医学研究科外科学系専攻 | ||||||||||
| 版 | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学 | |||||||||
| 書誌情報 |
博士学位論文 en : Thesis or Dissertation p. 1-29, 発行日 1992-01-01 |
|||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | [ABSTRACT] Fibronectin has been reported to play an essential role in the attachmentand migration of the corneal epithelial cells and has been successfully appliedfor the treatment of non - healing corneal epithelial disorders. Cornealepithelial cells have been suggested to possess fibronectin receptors on theircell surfaces. To investigate the role of fibronectin receptors during the woundhealing of the cornea, chronological changes in the localization of fibronectinreceptors were examined. The effects of the cell-adhesion- related peptideGRGDSP, an amino acid sequence of the cell binding domain of fibronectin,on the epithelial migration, and the effects of epidermal growth factor (EGF)on the regulation of fibronectin receptors activity were also studied. Byimmunofluorescence microscopy, the fibronectin receptors were observedpredominantly in the basal cell layer and they were detected to a lesser degree in wing cell layer of the corneal epithelium. Shortly after an incision was madeinto the cornea, fibronectin receptors were observed in all the cell layers ofthe migrating epithelium apparently to cover the defects. When the cornea hadhealed, they were only observed in the basal layer strongly and in the wingcell layer weakly as in the normal cornea. The appearance and disappearanceof fibronectin receptors in the corneal epithelial cells coordinated well withthat of fibronectin which formed a temporary extracellular matrix. Thusfibronectin receptors and fibronectin seem to be very important for successfulcorneal epithelial wound healing. To further clarify the dynamic role offibronectin receptors during epithelial wound healing, the effects of GRGDSPon the epithelial migration and attachment were studied. GRGDSP inhibitedthe epithelial migration on the organ- cultured cornea, fibronectin- stimulatedepithelial migration, and epithelial cell attachment to the fibronectin matrix.These findings suggest a very important role of fibronectin receptors in themigration and attachment of corneal epithelial cells. EGF is known toaccelerate corneal epithelial wound healing. EGF stimulated not only theepithelial migration on the organ- cultured cornea but also increased thenumber of cells attached to the fibronectin matrix. EGF- stimulated epithelialmigration and cell attachment to the fibronectin matrix were both inhibitedby GRGDSP. These findings strongly suggest that EGF stimulates theexpression of fibronectin receptors in the corneal epithelial cells, and that afibronectin-fibronectin receptor system plays a central role in the epithelialmigration of the cornea, which leads to epithelial wound healing. | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 学位の種類：医学 学位授与年月日：1992/3/19 指導： 大鳥, 利文 教授(Director: Prof. Otori, Toshifumi) 報告番号：甲第215号 学内授与番号：医甲168 NDL書誌ID：000000266473 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
