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Apoptosis in the Degeneration Process of Unfertilized Mouse Ova
https://kindai.repo.nii.ac.jp/records/2120
https://kindai.repo.nii.ac.jp/records/21205312e68a-1324-4ba5-a0a9-a85992db5514
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Thesis_Mek0264-19950325-0001.pdf (2.2 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2014-01-20 | |||||||||
| タイトル | ||||||||||
| タイトル | Apoptosis in the Degeneration Process of Unfertilized Mouse Ova | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | マウス未受精卵の変性過程におけるアポトーシスの検討 | |||||||||
| 著者 |
Takase, Kikuya
× Takase, Kikuya
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | ovum, polar body, apoptosis, fertilization, parthenogenesis | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||||
| 資源タイプ | thesis | |||||||||
| 著者(英) | ||||||||||
| en | ||||||||||
| 高瀬, 規久也 | ||||||||||
| 著者 所属 | ||||||||||
| Graduate School, Kinki University Course of Medicine Department of Obstretricand Gynecology | ||||||||||
| 著者所属(翻訳) | ||||||||||
| 近畿大学大学院 医学研究科 外科学系専攻 | ||||||||||
| 版 | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学 | |||||||||
| 書誌情報 |
博士学位論文 en : Thesis or Dissertation p. 1-16, 発行日 1995-03-01 |
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | [Abstract] In mammals, ova which are not fertilized undergo degeneration. Thus, it seems that the ovum is programmed to die unless fertilization andembryogenesis occurs, but little is known about the mechanism of such degeneration. To investigate this process, we observed the morphologicalchanges of cultured unfertilized ova and stained DNA fragmentation by the modified TUNEL method (treating floating samples in liquid reagents) todetect apoptosis. Ova were collected from the oviducts of superovulated mice and cultured for observation. The number of morphologicallyabnormal ova with shrinkage of the ooplasm and cytoplasmic fragmentation, which are typical features of apoptosis, showed a significant grandual increase from 24 to 32 hours (p＜0.05) and an abrupt increase from 40 hr of incubation (P＜0.001) . DNA fragmentation, which is one of the biological changes seen in apoptosis, was observed in the ooplasm of both intact and abnormal ova, cells with cytoplasmic fragmentation, and in the first polar body at the time of ovum collection as well as after incubation. These findings demonstrate that apoptosis is related to the process of degeneration in the mouse ovum and first polar body. | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 学位の種類：医学 学位授与年月日：1995/3/25 指導： 野田, 起一郎 教授(Director: Prof. Noda, Kiichiro) 報告番号：甲第322号 学内授与番号：医甲264 NDL書誌ID：000000286590 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
