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Two distinct nuclear factors, Sp 1 and RBF- 1, maintaining constitutive promoter activity of human angiotensinogen gene
https://kindai.repo.nii.ac.jp/records/2115
https://kindai.repo.nii.ac.jp/records/2115ba26a7cd-d6cf-49c4-b554-3a8c0d0e6771
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Thesis_Me0467-19960325-0001.pdf (3.9 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2014-01-20 | |||||||||
| タイトル | ||||||||||
| タイトル | Two distinct nuclear factors, Sp 1 and RBF- 1, maintaining constitutive promoter activity of human angiotensinogen gene | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | ヒトアンジオテンシノーゲン遺伝子5'-プロモーター転写活性化領域の解析 | |||||||||
| 著者 |
Shimada, Seijiro
× Shimada, Seijiro
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | Human Angiotensinogen, Gene regulation, Sp 1, RBF-1, HepG 2 cells | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||||
| 資源タイプ | thesis | |||||||||
| 著者(英) | ||||||||||
| en | ||||||||||
| 島田, 誠二郎 | ||||||||||
| 著者 所属 | ||||||||||
| Graduate School of Medicine, Kinki University Course of Medicine First Department of Internal | ||||||||||
| 著者所属(翻訳) | ||||||||||
| 近畿大学大学院 医学研究科 内科学系専攻 | ||||||||||
| 版 | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学 | |||||||||
| 書誌情報 |
博士学位論文 en : Thesis or Dissertation p. 1-20, 発行日 1996-03-01 |
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | [ABSTRACT] Angiotensinogen, the protein precursor of angiotensin II which is a critical regulator of blood pressure and electrolyte balance, is constitutively produced by the liver and secreted into the circulation. The 5 '-flanking region of the human angiotensinogen gene has been found to regulate its own expression in the liver of transgenic mice, but the transcriptional regulation of the human angiotensinogen gene has not been reported. We analyzed its promoter region by utilizing the reporter plasmids that contain the 5 '-flanking region with various lengths fused to the bacterial chloramphenicol acetyltransferase (CAT) gene. In transiently transfected human hepatoma HepG 2 cells, the only 32-bp DNA region located at nucleotide positions between -344 and -313 by was shown to mediate half as much as the full extent of parental promoter activity. An electrophoretic mobility shift assay (EMSA) using this segment identified the formation of DNA/HepG 2 nuclear factor complexes. These complexes could be dissociated by competing recognition sequences that contain those of either Sp 1 , a widely expressed GC box-binding factor, or RBF- 1 , a recently characterizedGC box-binding factor regulating the human retinoblastoma (Rb) gene. An in vivo competition experiment also suggested that the CAT activity derived from the cotransfected angiotensinogen promoter is reduced by a competitor containing RBF- 1 binding sites or containing Sp 1 binding sites. These findings suggest that Sp 1 and RBF- 1 play an important role in maintaining the constitutive promoter activity of the human angiotensinogen gene and that the angiotensinogen gene is characterized as the second target for RBF- 1 beside the Rb gene. | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 学位の種類：医学 学位授与年月日：1996/3/25 指導： 香取, 瞭 教授(Director: Prof. Katori, Ryo) 報告番号：甲第349号 学内授与番号：医467 NDL書誌ID：000000296578 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
