{"created":"2023-06-20T16:49:01.274279+00:00","id":21094,"links":{},"metadata":{"_buckets":{"deposit":"1cca3338-1416-4bf0-8e37-03ea3f377320"},"_deposit":{"created_by":3,"id":"21094","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"21094"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00021094","sets":["14:923:1809:4605"]},"author_link":["36026","36027","36028","36029"],"control_number":"21094","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"31","bibliographicPageEnd":"20","bibliographicPageStart":"13","bibliographic_titles":[{"bibliographic_title":"かやのもり：近畿大学産業理工学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Reports of Faculty of Humanity-Oriented Science and Engineering, Kindai University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For clinical applications of small interfering RNA (siRNA), chemical structure of siRNA should be optimized to be taken up into cells effectively, be resistant against nuclease digestion, be loaded onto RNA induced silencing complex (RISC) rapidly and correctly, bind to the target sequence of mRNA specifically, suppress the genetic expression efficiently and minimize off-target effects. In the present study, we investigated RNA interference (RNAi) efficiencies of siRNAs bearing 5’-O-methylthymidine (X) and 5’-aminothymidine (Z) at 5’-end of the strands. The results showed that X and Z at the 5’-end of the antisense strand gave a serious damage to siRNA to show almost no or largely reduced silencing effect. The results can be interpreted that RISC was destabilized by steric or electrostatic repulsion between the methyl group of X or the ammonium group of Z and the cationic residues in MID domain pocket of hAgo2. These results strongly suggested that modification of 5’-end of the sense strand with X and Z will eliminate an off-target effect of the strand.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15100/0000021094","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学産業理工学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13495801","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"琉球大学熱帯生物圏研究センター"},{"subitem_text_value":"近畿大学大学院産業理工学研究科"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科;教授"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科;教授"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"琉球大学","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"塩浜, 康雄","creatorNameLang":"ja"},{"creatorName":"シオハマ, ヤスオ","creatorNameLang":"ja-Kana"},{"creatorName":"Shiohama, Yasuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"36026","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤田, 崇史","creatorNameLang":"ja"},{"creatorName":"フジタ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujita, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"36027","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"神武, 洋二郎","creatorNameLang":"ja"},{"creatorName":"コウタケ, ヨウジロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kotake, Yojiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"36028","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤井, 政幸","creatorNameLang":"ja"},{"creatorName":"フジイ, マサユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"36029","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-22"}],"displaytype":"detail","filename":"AA12013209-20200630-0013.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA12013209-20200630-0013.pdf","url":"https://kindai.repo.nii.ac.jp/record/21094/files/AA12013209-20200630-0013.pdf"},"version_id":"ad791928-2d0c-4a59-b4ce-dfec1b939de7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"siRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"off-target effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"strand selection","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chemical modification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hAgo2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RISC","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈論文・報告〉センス鎖によるオフターゲット効果を低減するsiRNA の化学修飾","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈論文・報告〉センス鎖によるオフターゲット効果を低減するsiRNA の化学修飾","subitem_title_language":"ja"},{"subitem_title":"〈PAPERS and REPORTS〉Chemical Modification of siRNA Eliminating Off-target Effect by the Sense Strand","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["4605"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-09-22"},"publish_date":"2020-09-22","publish_status":"0","recid":"21094","relation_version_is_last":true,"title":["〈論文・報告〉センス鎖によるオフターゲット効果を低減するsiRNA の化学修飾"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-15T06:00:39.101596+00:00"}