{"created":"2023-06-20T16:48:55.445096+00:00","id":20952,"links":{},"metadata":{"_buckets":{"deposit":"5011544f-4f9b-45f3-8719-7ccf9e128ee2"},"_deposit":{"created_by":3,"id":"20952","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20952"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020952","sets":["14:923:2219:4588"]},"author_link":["35742","35743"],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"25","bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kindai University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要旨]生体内のタンパク質には、会合体を形成して働くものが多くある。また、正常な天然構造とは異なる、異常なコンフォメーションにミスフォールドし、病気に関わるアミロイド線維を形成することもある。これらの会合体の形成と解離は、タンパク質の構造状態変化のひとつである。圧力はタンパク質の構造状態を変化させることが分かつている。さらに、その変化から種々の熱力学的特徴が調べられる。今回我々は、βラクトグロプリン(βLG) の二量体会合系とαシヌクレイン(αSN) のアミロイド線維という異なる種類のタンパク質会合体の解離会合への圧力の影響をNMRを用いて調べた。これらに圧力印加したところ、αSNのアミロイド線維の解離は観測されたものの、βLGダイマーの解離会合平衡には影響が見られなかった。このような違いが見られた理由として(1)力学的圧縮による単量体構造の歪みの会合体全体への影響、(2)会合面の残基の水和体積の変化、(3)解離会合に伴う溶媒排除体積変化の違い、の寄与について考察する。","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"TOMIYAMA,Ryousuke"},{"subitem_text_language":"en","subitem_text_value":"SAKURAI,Kazumasa"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先大学悦生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学先大学悦生物理工学研究科生物工学専攻: 近畿大学先端技術総合研究所高圧力蛋白質研究センター "}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Major in Biotechnological Science,Graduate School of Biology-Oriented Science and Technology, Kindai University"},{"subitem_text_value":"Major in Biotechnological Science,Graduate School of Biology-Oriented Science and Technology, Kindai University: Advanced Technology Research Institute,Kindai University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"冨山, 涼介"},{"creatorName":"トミヤマ, リョウスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"櫻井,  一正"},{"creatorName":"サクライ, カズマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-22"}],"displaytype":"detail","filename":"AA11574630-20200331-0001.pdf","filesize":[{"value":"9.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20200331-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/20952/files/AA11574630-20200331-0001.pdf"},"version_id":"85af3290-78fc-49fa-a3e3-59d592f67cb2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"圧力","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク賀","subitem_subject_scheme":"Other"},{"subitem_subject":"自己会合","subitem_subject_scheme":"Other"},{"subitem_subject":"βラクトグロプリン","subitem_subject_scheme":"Other"},{"subitem_subject":"αシヌクレイン","subitem_subject_scheme":"Other"},{"subitem_subject":"high-pressure NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"self-assembly","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"β-lactoglobulin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"α-synuclein","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈Original Paper〉タンパク質会合状態に対する圧力効果の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈Original Paper〉タンパク質会合状態に対する圧力効果の検討"},{"subitem_title":"The pressure effect on the assemblies of protein molecules investigated by high-pressure NMR observation","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["4588"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-22"},"publish_date":"2020-06-22","publish_status":"0","recid":"20952","relation_version_is_last":true,"title":["〈Original Paper〉タンパク質会合状態に対する圧力効果の検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:19:18.875597+00:00"}