{"created":"2023-06-20T16:48:42.249275+00:00","id":20718,"links":{},"metadata":{"_buckets":{"deposit":"71057a85-d392-468a-b804-b72ef5f3b7c4"},"_deposit":{"created_by":3,"id":"20718","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20718"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020718","sets":["14:2667:4550"]},"author_link":["3854","43088"],"control_number":"20718","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2018)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K15026/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：現在のところ、異種間体細胞核移植による個体作製はほとんど成功しない。そこで本研究では、卵核胞期卵母細胞に異種の体細胞を移植して成熟させることで異種間核移植成熟卵子を獲得し、それを用いて異種間核移植の成功率を向上させることが可能かどうかを検討した。モルモット線維芽細胞をマウスおよびブタ卵核胞期卵母細胞に移植して体外成熟させ、第一極体を放出させることには成功したが、通常の成熟卵子でみられるような紡錘体はみられなかった。また、これらを活性化させて単為発生させた場合においても、これらの染色体を通常の成熟卵子に核移植した場合においても、2細胞期以降へ発生することはほとんどなかった。研究成果の概要（英文）：Successful production on animals by interspecies nuclear transfer (iNT) is extremely rare. In this study, we attempted to improve iNT by using germinal vesicle (GV) stage oocytes. Guinea pig fibroblasts were transferred into mouse and porcine GV oocytes, and those iNT oocytes extruded the first polar body after in vitro maturation. However, those iNT oocytes didn't have the normal-shaped spindle, indicating that they were not \"true\" matured oocytes. The iiNT oocytes failed to develop beyond 2-cell stage after parthenogenetic activation, and the chromosomes of iNT oocytes didn't show the developmental potential beyond 2-cell stage after transfer into normal matured oocytes. In conclusion, our novel iNT method using GV oocytes didn't improve the development of iNT embryos.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：挑戦的萌芽研究;  研究期間：2016～2018;   課題番号：16K15026;   研究分野：発生工学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"},{"subitem_text_language":"en","subitem_text_value":"KUROSAKA, Satoshi"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 教授"},{"subitem_text_value":"近畿大学教授先端技術総合研究所; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"細井, 美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"黒坂, 哲"},{"creatorName":"クロサワ, サトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-12"}],"displaytype":"detail","filename":"16K15026seika.pdf","filesize":[{"value":"281.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"16K15026seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/20718/files/16K15026seika.pdf"},"version_id":"550c88d8-c1ac-4750-ac5f-70bdca5cd28f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"発生工学","subitem_subject_scheme":"Other"},{"subitem_subject":"卵核胞期卵母細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"異種間核移植","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"卵核胞期卵母細胞を用いた新しい異種間体細胞核移植法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"卵核胞期卵母細胞を用いた新しい異種間体細胞核移植法の開発","subitem_title_language":"ja"},{"subitem_title":"Novel Interspecies Nuclear Transfer Using Germinal Vesicle Stage Oocytes.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4550"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-12"},"publish_date":"2020-03-12","publish_status":"0","recid":"20718","relation_version_is_last":true,"title":["卵核胞期卵母細胞を用いた新しい異種間体細胞核移植法の開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-21T05:14:34.986152+00:00"}