{"created":"2023-06-20T16:48:41.223809+00:00","id":20698,"links":{},"metadata":{"_buckets":{"deposit":"9870f988-3959-4555-b6c4-8706868cbb27"},"_deposit":{"created_by":3,"id":"20698","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20698"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020698","sets":["14:2667:4550"]},"author_link":["43063","3214"],"control_number":"20698","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2018)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K10958/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：免疫系への作用メカニズムをマクロファージTHP-1を用いてミダゾラムの作用メカニズムを検討した。ベンゾジアゼピンの作用分子の一つとしてTranslocator protein(TSPO)が知られている。THP-1をLPSで刺激すると炎症メディエーターと表面抗原の発現増加が起こり、ベンゾジアゼピンがこれを抑制するという系を用いて解析した。TSPOリガンドはベンゾジアゼピンと同様の抑制作用を示した。またミダゾラムはTHP=1のNF-kBとMAPKの活性化を抑制していた。TSPOを過剰発現した細胞とTSPOの発現を抑制した細胞を用いた解析からミダゾラムの作用にTSPOの関与があることが示された。研究成果の概要（英文）：We examined the immunomodulatory effects of the benzodiazepine midazolam on human macrophages and associated molecular mechanisms. We analyzed effects of midazolam pretreatment on LPS-induced upregulation of the costimulatory molecule and the pro-inflammatory factors in THP-l and in PMDMs. The effects of midazolam on NF-κB, and MAPK activation were analyzed in THP-1 cells. The role of TSPO was investigated using THP-1 cells overexpressing TSPO and with TSPO knockdown through transfection with small interfering RNA for TSPO. Results Midazolam suppressed upregulation of CD80 and release of IL-6 and NO in THP-1 cells and PMDMs. Midazolam suppressed the activation of NF-κB/AP-1 and MAPKs in human THP-1 cells. Macrophages overexpressing TSPO exhibited enhanced susceptibility to immunosuppression by midazolam, and macrophages lacking TSPO expression exhibited reduced effects of midazolam. Midazolam inhibits LPS-stimulated immune responses in human macrophages by activating TSPO signaling.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2016～2018;   課題番号：16K10958;   研究分野：周術期管理医学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Ohta, Noriyuki"},{"subitem_text_language":"en","subitem_text_value":"Fujino, Yuji"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 准教授"},{"subitem_text_value":"大阪大学大学院医学系研究科"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大田, 典之"},{"creatorName":"オオタ, ノリユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"藤野, 裕士"},{"creatorName":"フジノ, ユウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-12"}],"displaytype":"detail","filename":"16K10958seika.pdf","filesize":[{"value":"259.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"16K10958seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/20698/files/16K10958seika.pdf"},"version_id":"f227fb8f-1d4d-4923-ad81-0a85f24bd978"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ベンゾジアゼピン","subitem_subject_scheme":"Other"},{"subitem_subject":"TSPO","subitem_subject_scheme":"Other"},{"subitem_subject":"GABA","subitem_subject_scheme":"Other"},{"subitem_subject":"サイトカイン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ベンゾジアゼピンによる感染症予後悪化機構の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ベンゾジアゼピンによる感染症予後悪化機構の解明","subitem_title_language":"ja"},{"subitem_title":"The mechanism of worsening effects of benzodiazepine on immune defense","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4550"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-12"},"publish_date":"2020-03-12","publish_status":"0","recid":"20698","relation_version_is_last":true,"title":["ベンゾジアゼピンによる感染症予後悪化機構の解明"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-21T02:11:26.023318+00:00"}