{"created":"2023-06-20T16:48:40.822357+00:00","id":20692,"links":{},"metadata":{"_buckets":{"deposit":"abc3ee97-bd4f-4e99-a2ca-f0fa95acffd9"},"_deposit":{"created_by":3,"id":"20692","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20692"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020692","sets":["14:2667:4550"]},"author_link":["3710","33594"],"control_number":"20692","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2018)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K09705/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究はERBB4を介する筋萎縮性側索硬化症(ALS)発症機序解明と抗がん剤の標的であるリン酸化カスケードを、ALS患者人工多能性幹(iPS)細胞由来神経細胞や神経様培養細胞で検討する事を目的とした。神経系細胞を用いた発現実験の結果、野生型と4種の変異型ERBB4の細胞内分布には大差がなかった。変異蛋白のほとんどは不安定であったが、リン酸化能は様々であった。抗がん剤は、ERBB4のリン酸化は抑制したが細胞障害性が強かった。患者iPS細胞由来通常神経細胞は、その維持が困難であったが、ラパマイシンやレチノイン酸が一部細胞死を抑制した。運動ニューロンへの分化でも、患者由来細胞は細胞死が誘導された。 研究成果の概要（英文）：This study was aimed to clarify the pathomechanism of amyotrophic lateral sclerosis (ALS) via an ERBB4-related kinase pathway and the effectiveness of anti-cancer kinase inhibitors, using ALS-patient-derived induced pluripotent stem (iPS) cells and cultured neuronal SH-SY5Ycells. In SH-SY5Y cells expressing wild-type (WT) and mutant ERBB4, subcellular localization did not differ between WT and mutant proteins. Most mutants were unstable, but kinase activities varied considerably between mutants. Anti-cancer drugs reduced phosphorylation of ERBB4, but exhibited cellular toxicity. The maintenance of regular neurons induced from patient-derived iPS cells were difficult, but rapamycin and retinoic acid reduced cell death. Motor neurons from patient-derived iPS cells were unstable during motor neuron induction.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2016～2018;   課題番号：16K09705;   研究分野：臨床神経遺伝学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"HIRANO, Makito"},{"subitem_text_language":"en","subitem_text_value":"SAIGOH, Kazumasa"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 准教授"},{"subitem_text_value":"近畿大学理工学部; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"平野, 牧人"},{"creatorName":"ヒラノ, マキト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"西郷, 和真"},{"creatorName":"サイゴウ, カズマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-12"}],"displaytype":"detail","filename":"16K09705seika.pdf","filesize":[{"value":"416.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"16K09705seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/20692/files/16K09705seika.pdf"},"version_id":"085dc45e-3d7a-404f-9514-db90d171ad3d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"リン酸化阻害剤","subitem_subject_scheme":"Other"},{"subitem_subject":"筋萎縮性側索硬化症","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"筋萎縮性側索硬化症の原因遺伝子ERBB4を介する発症機序解明と抗がん剤の効果検証","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"筋萎縮性側索硬化症の原因遺伝子ERBB4を介する発症機序解明と抗がん剤の効果検証","subitem_title_language":"ja"},{"subitem_title":"Clarification of pathomechanism via amyotrophic lateral sclerosis-causing ERBB4 and effectiveness of anti-cancer medicine.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4550"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-12"},"publish_date":"2020-03-12","publish_status":"0","recid":"20692","relation_version_is_last":true,"title":["筋萎縮性側索硬化症の原因遺伝子ERBB4を介する発症機序解明と抗がん剤の効果検証"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-20T07:55:38.830575+00:00"}