{"created":"2023-06-20T16:48:40.559222+00:00","id":20687,"links":{},"metadata":{"_buckets":{"deposit":"38cb0a6a-682f-490d-8ad0-b42e03aaa563"},"_deposit":{"created_by":3,"id":"20687","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20687"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020687","sets":["14:2667:4550"]},"author_link":["3387","30493"],"control_number":"20687","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2018)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K08821/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：APOBEC3は自身の酵素活性を介する機構と介さない機構の両面からウイルス複製を阻害するが、その分子機構の全容は解明されていない。本研究ではAPOBEC3がどのようにHIV-1とMuLVの増殖を阻害するのか、機序解明に取り組んだ。その結果、APOBEC3はウイルス酵素群の前駆体に結合し、その多段階的な切断を経て生じるウイルスプロテアーゼの産生を阻害することで、最終的にウイルス粒子の感染性を低下させると分かった。本活性はAPOBEC3のデアミナーゼ活性に非依存的であり、異常な前駆体切断を惹起するものであった。また、ウイルス粒子形成阻害を介してウイルスの放出量を低下させることも判明した。研究成果の概要（英文）：APOBEC3 is a cytidine deaminase and inhibits the replication of various　types of viruses through the deaminase-dependent and -independent ways. Understanding the entire　mechanisms that underlie the anti-viral function of APOBEC3 remains incomplete. Here, we　investigated how APOBEC3 interferes with the replication of human immunodeficiency virus (HIV-1) and　murine leukemia viruses (MuLVs). We found that APOBEC3 binds Gag-Pol precursor polyprotein and　interferes with the production of mature viral protease (PR) autoprocessed from the Gag-Pol　precursor, leading to an increase in immature virus particles, which can not infect with cells. Our　results suggest that this inhibitory effect of APOBEC3 is independent on its deaminase activity and　probably mediated through the promotion of aberrant digestion of Gag-Pol. We also found that APOBEC3　may reduce virion production likely through the binding to Gag-Pol and subsequent inhibition of　virion assembly.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2016～2018;   課題番号：16K08821;   研究分野：ウイルス学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"HAKATA, Yoshiyuki"},{"subitem_text_language":"en","subitem_text_value":"MIYAZAWA, Masaaki"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 講師"},{"subitem_text_value":"近畿大学医学部; 教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"博多, 義之","creatorNameLang":"ja"},{"creatorName":"ハカタ, ヨシユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"宮澤, 正顕","creatorNameLang":"ja"},{"creatorName":"ミヤザワ, マサアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-12"}],"displaytype":"detail","filename":"16K08821seika.pdf","filesize":[{"value":"94.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"16K08821seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/20687/files/16K08821seika.pdf"},"version_id":"8786c46c-64c5-48f5-bc66-ce2fb0b46e82"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Retrovirus","subitem_subject_scheme":"Other"},{"subitem_subject":"MuLV","subitem_subject_scheme":"Other"},{"subitem_subject":"HIV-1","subitem_subject_scheme":"Other"},{"subitem_subject":"APOBEC3","subitem_subject_scheme":"Other"},{"subitem_subject":"感染抵抗性因子","subitem_subject_scheme":"Other"},{"subitem_subject":"Gag-Pol","subitem_subject_scheme":"Other"},{"subitem_subject":"autoprocessing","subitem_subject_scheme":"Other"},{"subitem_subject":"Gag assembly","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"APOBEC3によるGag-Pol前駆体プロセシング阻害の機序解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"APOBEC3によるGag-Pol前駆体プロセシング阻害の機序解明","subitem_title_language":"ja"},{"subitem_title":"Molecular mechanism of APOBEC3-mediated inhibition on Gag-Pol autoprocessing","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4550"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-12"},"publish_date":"2020-03-12","publish_status":"0","recid":"20687","relation_version_is_last":true,"title":["APOBEC3によるGag-Pol前駆体プロセシング阻害の機序解明"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-20T07:36:59.218751+00:00"}