{"created":"2023-06-20T16:48:38.934278+00:00","id":20652,"links":{},"metadata":{"_buckets":{"deposit":"0805f635-66ce-4c98-879d-3821a029d171"},"_deposit":{"created_by":3,"id":"20652","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20652"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020652","sets":["14:2667:4550"]},"author_link":["4143","43167"],"control_number":"20652","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2018)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14652/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：真菌のキャッピング酵素Ceg1、Cet1および、Ceg1と結合できないCet1変異体を用いて、核移行シグナルはCet1のみに存在し、Ceg1はCet１に結合しないと核移行できないこと、細胞質でCet1とCeg1が結合し、Cet1-Ceg1複合体が核に移行することを明らかにしている。そして、酵母を用いた遺伝学的研究により、Cet1とCeg1の結合が真菌の生育に必須であることが示された。このことから、Cet1-Ceg1結合阻害が新規抗真菌薬の作用機序となりうる事が示された。そして、これまでにモデルとした出芽酵母のcapping反応を選択的に阻害するアプタマーを取得している。研究成果の概要（英文）：Using the fungal capping enzymes Ceg1, Cet1 and Cet1 mutants that can not bind to Ceg1, we show that nuclear translocation signals are present only in Cet1, and that Ceg1 can not translocate to the nucleus without binding to Cet1. Furthermore, we demonstrate that Cet1 and Ceg1 bind in the cytoplasm, and that the Cet1-Ceg1 complex translocates to the nucleus. Moreover, genetic studies using yeast show that Cet1 and Ceg1 binding is essential for fungal growth. These data indicate that inhibition of Cet1-Ceg1 binding may be the mechanism of action of novel antifungal agents. Then, we obtained the aptamers that selectively inhibit the capping reaction of budding yeast that has been used as a model so far.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：挑戦的萌芽研究;  研究期間：2016～2018;   課題番号：16K14652;   研究分野：RNA生化学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究協力者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"FUJIWARA, Toshinobu"},{"subitem_text_language":"en","subitem_text_value":"YAMASAKI, manabu"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学薬学部; 教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤原, 俊伸"},{"creatorName":"フジワラ, トシノブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"山﨑, 学"},{"creatorName":"ヤマサキ, マナブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-12"}],"displaytype":"detail","filename":"16K14652seika.pdf","filesize":[{"value":"559.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"16K14652seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/20652/files/16K14652seika.pdf"},"version_id":"ae9a7eb2-878a-4204-a0e5-5d5fb8b3ae35"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"RNAアプタマー","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"新規抗真菌薬創製を目指したRNA アプタマーの創製","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新規抗真菌薬創製を目指したRNA アプタマーの創製","subitem_title_language":"ja"},{"subitem_title":"Creation of RNA aptamers aiming at the creation of new antifungal drug","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4550"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-12"},"publish_date":"2020-03-12","publish_status":"0","recid":"20652","relation_version_is_last":true,"title":["新規抗真菌薬創製を目指したRNA アプタマーの創製"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-19T07:05:06.530588+00:00"}