{"created":"2023-06-20T16:48:38.379422+00:00","id":20639,"links":{},"metadata":{"_buckets":{"deposit":"3489a682-8b32-47b7-8db4-f295028b9269"},"_deposit":{"created_by":3,"id":"20639","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20639"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020639","sets":["14:2667:4550"]},"author_link":["30670","30671","43154"],"control_number":"20639","item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2018)"}]}]},"item_8_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K05856/","subitem_description_type":"Other"}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：申請者は、細胞内に薬剤となるタンパク質や機能性ペプチドを安全にかつ効率的に運搬することによって、病気を治療することを目的にしている。今回申請者は、Nanogタンパク質とヘテロ二量体化ロイシンジッパーペプチド（LzK）を連結させた。また、細胞内運搬ペプチドとLzKの対となるペプチド（LzE）を連結させた。これらのペプチドを混ぜるとNanogタンパク質が細胞質内、さらには核膜内に運搬されて、Nanogの持つ機能をうまく発現させることができた。研究成果の概要（英文）：We aim to treat diseases by safely and efficiently delivering proteins and functional peptides into cells. Here, we conjugated the Nanog protein to a heterodimeric leucine zipper peptide (LzK). In addition, an cell-penetrating peptide was conjugated to LzE peptide that forms a hybrid with LzK. When these peptides were mixed, the Nanog protein was successfully delivered into the cytoplasm and further into the nucleus, and the function possessed by Nanog protein was successfully expressed.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2016～2018;   課題番号：16K05856;   研究分野：生体機能関連化学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ(WEKO2)","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"研究協力者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"KITAMATSU, Mizuki"},{"subitem_text_language":"en","subitem_text_value":"MICHIUE, Hiroyuki"},{"subitem_text_language":"en","subitem_text_value":"HAKATA, Yoshiyuki"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学理工学部; 准教授"},{"subitem_text_value":"岡山大学中性子医療研究センター; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"北松, 瑞生"},{"creatorName":"キタマツ, ミズキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"道上, 宏之"},{"creatorName":"ミチウエ, ヒロユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"博多, 義之"},{"creatorName":"ハカタ, ヨシユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-12"}],"displaytype":"detail","filename":"16K05856seika.pdf","filesize":[{"value":"474.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"16K05856seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/20639/files/16K05856seika.pdf"},"version_id":"350fbc41-61a3-4341-93c6-3fdd8bb78c6b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ペプチド","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞内運搬ペプチド","subitem_subject_scheme":"Other"},{"subitem_subject":"ペプチド核酸","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ペプチドジッパー法により細胞内運搬したタンパク質や機能性ペプチドの効果的機能改善","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ペプチドジッパー法により細胞内運搬したタンパク質や機能性ペプチドの効果的機能改善","subitem_title_language":"ja"},{"subitem_title":"Effective functional improvement of proteins and peptides delivered into cell by peptide zipper method","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4550"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-12"},"publish_date":"2020-03-12","publish_status":"0","recid":"20639","relation_version_is_last":true,"title":["ペプチドジッパー法により細胞内運搬したタンパク質や機能性ペプチドの効果的機能改善"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-15T04:17:48.239942+00:00"}