{"created":"2023-06-20T16:48:15.865678+00:00","id":20092,"links":{},"metadata":{"_buckets":{"deposit":"af31dfee-72cc-46eb-9801-85f2333ea9b5"},"_deposit":{"created_by":3,"id":"20092","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"20092"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00020092","sets":["14:923:2219:4501"]},"author_link":["34209"],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"24","bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kindai University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要旨]これまでの研究から、圧力の増加に伴い(ⅰ)力学的圧縮と(ⅱ)熱力学的転移の2つのタイプの蛋白質構造変化が同時に進行することが分かっている。(ⅰ)力学的圧縮とは蛋白質が天然状態や中間体状態などあるひとつの状態のまま圧縮される構造変化であるのに対し、(ⅱ)熱力学的転移とは異なる状態への転移に伴う構造変化のことである。高圧NMRという手法で各アミノ酸残基の化学シフトの圧力依存変化を測定し、それを主成分解析することで、(ⅰ)力学的圧縮と(ⅱ)熱力学的転移に関わるアミノ酸残基を分離して同定できる。筆者は以前、βラクトグロブリン(βLG)という蛋白質に対しこの解析を行ったところ、(ⅱ)熱力学的転移を示す残基が、折り畳み初期中間体のコア領域の残基と一致することが分かり、折り畳み過程の知見を得るうえでこの解析法は有用であることを示した。今回は、折り畳み経路が変化した変異体について同様の高圧NMR解析を行い、野生型と比較した。その結果、変異体の方ではコア領域がβLGのもつ2枚のβシートに渡って分布することが分かった。以前の折り畳み研究から、この変異体はシート間が相互作用することによって折り畳み効率が悪くなることが示唆されており、今回の解析結果はそれと一致するものであった。\nAccording to the previous studies,the following two types of conformational changes of protein occur upon increase in pressure: That is, (ⅰ)mechanical compression and (ⅱ) transition between thermodynamically distinct states.Pressure-dependent chemical shift changes of individual residues can be measured by using high-pressure NMR apparatus. A principal component analysis can decompose the obtained data into the two types of conformational changes. The author previously applied this method to β-lactoglobulin (βLG) and found that the residues which are associated with the conformational change of  (ⅱ) agreed well with those which were included in the formation of core regions in the early step of the folding process. Thus,the high-pressure NMR method is suggested to be a potential method to identify the core region of the folding intermediate of protein molecule. In order to assess this suggestion, the author examined the same method to the E44L βLG mutant, which folds in a different route from that of the wild type. As a result of the analysis, the residues responsible for the conformational change of (ⅱ) were found to be distributed on the both β-sheets in the native structure of βLG, indicating that these two β-sheets are interacting with each other in the early step in its folding process.The previous study indicated that the folding process of the mutant is perturbed due to the interaction between the two β-sheets. Thus, the present conclusion is consistent with that of the previous study, confirming the versatility of the method.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"SAKURAI,Kazumasa"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先端技術総合研究所高圧力蛋白質研究センター "}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"High Pressure Protein Research Center, Institute of Advanced Technology, Kindai University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"櫻井, 一正"},{"creatorName":"サクライ, カズマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-18"}],"displaytype":"detail","filename":"AA11574630-20190331-0001.pdf","filesize":[{"value":"7.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20190331-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/20092/files/AA11574630-20190331-0001.pdf"},"version_id":"514d891d-8fe2-44d6-9fe4-ab3d8233d15b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"圧力NMR","subitem_subject_scheme":"Other"},{"subitem_subject":"蛋白質の折り畳み","subitem_subject_scheme":"Other"},{"subitem_subject":"βラクトグロブリン","subitem_subject_scheme":"Other"},{"subitem_subject":"化学シフト","subitem_subject_scheme":"Other"},{"subitem_subject":"主成分解析","subitem_subject_scheme":"Other"},{"subitem_subject":"high-pressure NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein folding","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"β-lactoglobulin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chemical shift","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"principle component analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈Original Papers〉圧力NMR によるβラクトグロブリン変異体の折り畳み初期中間状態における残基間相互作用の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈Original Papers〉圧力NMR によるβラクトグロブリン変異体の折り畳み初期中間状態における残基間相互作用の解析"},{"subitem_title":"Identification of inter-residue interactions appeared in the early stage of folding process of β-lactoglobulin and its folding mutant, E44L, by using high-pressure NMR","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["4501"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-18"},"publish_date":"2019-04-18","publish_status":"0","recid":"20092","relation_version_is_last":true,"title":["〈Original Papers〉圧力NMR によるβラクトグロブリン変異体の折り畳み初期中間状態における残基間相互作用の解析"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:30:57.595024+00:00"}